Slowing of axonal regeneration is correlated with increased axonal viscosity during aging
© Lamoureux et al; licensee BioMed Central Ltd. 2010
Received: 1 June 2010
Accepted: 25 October 2010
Published: 25 October 2010
As we age, the speed of axonal regeneration declines. At the biophysical level, why this occurs is not well understood.
To investigate we first measured the rate of axonal elongation of sensory neurons cultured from neonatal and adult rats. We found that neonatal axons grew 40% faster than adult axons (11.5 µm/hour vs. 8.2 µm/hour). To determine how the mechanical properties of axons change during maturation, we used force calibrated towing needles to measure the viscosity (stiffness) and strength of substrate adhesion of neonatal and adult sensory axons. We found no significant difference in the strength of adhesions, but did find that adult axons were 3 times intrinsically stiffer than neonatal axons.
Taken together, our results suggest decreasing axonal stiffness may be part of an effective strategy to accelerate the regeneration of axons in the adult peripheral nervous system.
Following injury of peripheral nerves in adults, significant regeneration occurs but at a rate slower than in the young . For example, using radiotracer studies Pestronk et al.,  found the average rate of regeneration of rat sciatic sensory neurons occurs at a rate of ~2.6 mm/day in animals that are 2 mo old and slows to a rate of 0.3 mm/day in animals that are 28 mo old. Based on these numbers regeneration of a nerve with a length of 1 m could be accelerated from ~8 years to ~1 year if rates of regeneration found in younger animals could be achieved in adults. If we understand the mechanics of axonal elongation, it could be possible to devise strategies to speed regeneration of peripheral nerves from years to months, allowing the reinnervation of distal muscles before the occurrence of irreversible loss of muscle function [3, 4].
While the molecular influences (inhibitory proteins, growth factors, adhesion molecules, etc.) underlying poor regeneration of adult peripheral neurons have been extensively analyzed [5, 7], the intrinsic biophysical properties of individual neurons have only recently been investigated [8–14]. Our recent work using embryonic sensory neurons suggests that axonal elongation occurs through a two step process where forces at the growth cone stretch the axon and new material is added along the axonal shaft [15, 16]. Our mathematical modeling predicts the rate of axonal stretching/elongation is a function of the level of force generation at the growth cone, the strength of adhesions of the axon to the substrate, axonal diameter, and the mechanical stiffness (i.e. viscosity) of the axon . To determine if there are intrinsic biophysical differences that could explain the slow regeneration of adult sensory neurons, we used force calibrated towing needles to characterize the biophysical properties of neonate rat and adult rat axons. We found no difference in the adhesion levels between the neonate and adult rat neurons, but a significant difference in axonal viscosity that increased with developmental age.
The rate of axonal elongation decreases with aging
The rate of axonal regeneration decreases with age
# of growth cones
The axons of adult sensory neurons lengthen by stretching
Axonal viscosity increases during aging
Growth cone force generation is not reduced during aging
Axonal biophysical parameters as a function of age
G (g µm/hour)
G (g µm/hour)
G (g µm/hour)
g (g/µm hour)
g (g/µm hour)
g (g/µm hour)
Estimated force generation at the growth cone as a function of age
Velocity (g µm/h)
G (g µm/h)
F0 (g µm/h2)
Changes in sensory neuron growth cone size have been observed during embryonic development. In particular, growth cones from embryonic day 7 neurons grown on polylysine are 146% greater in size than neurons from embryonic day 14 neurons . To determine if growth cone size is changing between postnatal and adult neurons, we measured their area. We found the average area of the neonatal growth cones to be 46 +/- 51 µm2 (average +/- standard deviation, n = 53 growth cones) and the area of the adult growth cones to be 63 +/- 54 µm2(n = 46 growth cones). While there was a trend for adult growth cones to be larger, we did not find a significant difference in growth cone size using a two-tailed t-test.
As we age our bodies and minds become less flexible. Presumably, this is the result of changes that are occurring at the cellular level. To determine if there are intrinsic biophysical changes in individual neurons that could explain the slowing of axonal regeneration that occurs during aging, we used force calibrated towing needles to characterize the biophysical properties of neonate rat and adult rat axons. We chose this type of neuron because they have been extensively characterized in terms of growth and the molecular changes that occur during regeneration [1, 7, 17–21]. We found no significant differences in terms of axonal diameter, adhesion strength, or force generation by the growth cone, that could explain the slowing of axonal regeneration during aging. Yet we did find a significant increase in viscosity of the axons that increased with developmental age.
The conventional model of axonal elongation suggests that axonal elongation occurs primarily through a mechanism that involves the assembly of new axon at the tip of an otherwise stationary axonal framework [23, 24]. In contrast, our recent work on the rapidly growing (i.e. ~35 µm/hour) embryonic chick sensory neurons suggests a Stretch and Intercalation model which proposes axonal elongation occurs through a two step process where forces generated at the growth cone stretch the axon and new material is added along the axonal shaft [11, 15, 16]. At a biophysical level, there are two simple possibilities that could explain the slowing of axonal regeneration that occurs during aging. The first is that the rate of elongation differs because the mechanism of growth differs (e.g. tip growth vs. stretch and intercalation) . The second is that the mechanism of elongation is the same, but that rates of elongation differ because of quantitative differences in key parameters. To distinguish between these possibilities we stained the mitochondria along the axons and monitored the movement of the docked mitochondria during normal growth cone mediated axonal elongation (Figure 2). As we observed in the sensory neurons from embryonic animals , here we found that in neonatal and adult animals axonal elongation was coupled with axonal stretching, but with quantitative differences in the velocity profiles (Figure 2E). We take this as evidence that these neurons share the same basic mechanism of outgrowth but differ quantitatively in terms of their biophysical properties.
Based on our mathematical modeling, the slowing of elongation with aging could arise from an increase in the strength of adhesions (η) between the axons and the substrate, an increase in the diameter of the axons (d), an increase the intrinsic viscosity (g) of the axons, a decrease in the forces generated by the growth cones (F 0 ), or some combination of these variables. To experimentally test our predictions based on growth cone mediated axonal elongation, we applied known levels of forces to growth cones and analyzed the movement of docked mitochondria to measure the biophysical changes in the neurons that were occurring during aging. These experiments strongly suggested that the key factor was not an increase in the strength of adhesions (η), but rather an increase in total axonal viscosity (G). To determine if the increase in G was the result of a change in axonal diameter or intrinsic axonal viscosity, we measured axonal diameter. We found that neurons cultured from older animals had a significantly smaller axonal diameter, which indicates that the intrinsic viscosity (g) of the axons is increasing. Based on our measurements of average rate of axonal elongation, axonal viscosity (G), and adhesion strength (η), we estimated the force generation at the growth cone (F0) for sensory neurons cultured from the neonatal and adult rats. This formula suggested that force generation was similar in these two type of neurons (Table 3). Altogether, the most conservative interpretation of our data is that the primary factor limiting the rate of elongation in the adult neurons is their high intrinsic viscosity (g).
A caution to be noted in interpreting the adhesion data (η) is that our experiments were conducted on neurons grown on polyornithine. In vivo, laminin and chondroitin sulfate proeteoglycans are modulated through development and upregulated after peripheral nerve injury [25–28]. In addition the receptors for laminin, integrins, are developmentally regulated and when expressed increase regeneration following axotomy [29–32]. While we did not observe changes in adhesion strength for neurons grown on polyornithine as a function of age (Figure 3), such changes may be occurring. Further analysis involving systematic analysis of adhesion on physiologically relevant substrates is needed and may reveal significant differences in substrate adhesion during neuronal maturation.
As we have previously conducted a biophysical analysis of the elongation of chicken embryonic sensory neurons , it is of interest to compare the results published here with our previous work. Nonetheless it is important to keep in mind that differences between the properties of the chicken sensory neurons with the rat sensory neurons could be attributed to species or age. With these considerations, in our measurements of axonal viscosity we found both total axonal viscosity (G) and intrinsic axonal viscosity (g) increased substantially with the developmental age of the animal (Table 2). Statistical analysis between the published and new data, revealed significant difference between all three groups (p < 0.0001 for each, two-tailed t-tests). This suggests there is a progressive increase in axonal stiffness through the embryonic, neonatal, and adult developmental stages.
A previous analysis of the cellular changes that are occurring in growth cones during developmental maturation demonstrated that a significant (> 50%) decrease in sensory neuron growth cone size occurs between embryonic day 7 and 14 . This is correlated with a decrease in the sensitivity of axonal elongation to cytochalasin and an increase in the stability of the microtubule and neurofilament cytoskeletons. While we did not observe significant differences in growth cone size between neonatal and adult sensory neurons (46 +/- 51 µm2 vs. 63 +/- 54 µm2 respectively), our results are on balance consistent with the findings of Jones et al., . In particular, our observation that axonal viscosity increases with age could be explained by increased stability of neurofilaments, as they observed following treatment with cytochalasin; and/or reduced microtubule dynamics, as they observed based on levels of tyrosinated tubulin and EB3 comet velocity .
Our work is the first to examine the biophysical changes that occur in individual neurons during aging. We found adult axons grew ~30% slower and were 3 times intrinsically stiffer than neonatal axons. Taken together our results suggest targeted molecular approaches to decreasing axonal stiffness may be part of an effective strategy to accelerate the regeneration of axons in the adult peripheral nervous system.
Adult and neonate sensory neurons were cultured by a modification of a protocol developed by Lindsay et al., [17, 49], which uses a gentle enzymatic dissociation of the ganglion tissue. Supernumerary adult rats (> 200 g) were euthanized and their DRGs dissected and placed into Hanks Balanced Saline, without Ca2+ and Mg2+, buffered to pH 7.4 with 5 mM HEPES (HBSS-). After removal of ventral roots and ganglia capsules, the DRGs were dissociated for 10 minutes at 37°C in activated papain (Worthington Biochemical Corp, Lakewood, NJ) at a concentration of 50 U/ml in HBSS-. The ganglia from adult animals were transferred to a second enzyme solution containing 5 mg/ml dispase: 1 mg/ml collagenase (Life Technologies, Carlsbad, CA) in HBSS- for 10 minutes at 37°C, these enzymes were not used for the neonates. The ganglia were then triturated 10 times with a fire-polished Pasteur pipette, followed by another 10 min digestion and trituration in the same solution. Single cells were separated from larger chunks by gravity sedimentation. The single cells were then pelleted by brief centrifugation, and resuspended in culture medium for plating. Axonal outgrowth was supported in L-15 containing 10% fetal calf serum and 50 ng/ml 7S nerve growth factor (NGF) and N9 growth supplement  in plates treated with 0.01% polyornithine.
To track axonal stretching during normal axonal elongation and while towing, mitochondria were labeled with Mitotracker  and observed with a Leica DM IRB inverted microscope and observed with a N Plan L 40/0.55 corr Ph2 with an adjustable collar infinity/0 - 2/c objective. Cells were illuminated with a 100 W Xenon lamp attenuated 98% with neutral density filters through a Texas Red cube (Chroma, Rockingham, VT) for visualization of MitoTracker .
Images were taken with Openlab (Improvision, Waltham, MA) using an Orca-ER digital camera CCD, model #CA742-95 (Hamamatsu, Bridgewater, NJ), converted into TIFFs and analyzed using ImageJ (NIH) as previously described .
A biophysical model of axonal elongation
In brief, Eq. 2 gives the profile for velocity as a function of distance, x; axonal length, L; force at the growth cone, F o ; axonal viscosity, G; and adhesion strength, η. For the details on the derivation of this formula refer to .
Determination of axonal viscosity and adhesiveness
Axons were towed as previously described . In brief, force calibrated towing needles previously coated in polylysine (1 mg/ml) and concanavalin A (1 mg/ml) were used to apply forces to growth cones. Force measurements were acquired from phase images throughout the experiment. To track bulk movement along the axon in response to forces, fluorescent images of docked mitochondria were analyzed using ImageJ (NIH). To calculate G and η, lines were fitted to the velocity data in the kymographs to calculate the rate of change of the velocity of the mitochondria. Using force measurements from the calibrated needles, a value of was found by dividing the average force over this interval by the slope of the fitted line. Once values of were determined (one value of per 30 minute) the Origin software package (OriginLab Corporation, Northampton, MA) was used with Eq. 2 to fit the best value of η to the data. For this calculation the velocities of mitochondria proximal to the point of adhesion were used. Empirical values of F o ,L , and G were fixed and a Levenberg-Marquardt algorithm was implemented in the Origin package to find the optimal value of η. The relationship G = gA (A = cross-sectional area) was used to calculate the intrinsic axial viscosity g for each axon. Phase images of each trial were analyzed using ImageJ to determine the axonal diameter at various times. For each phase image, the diameter was measured along the axon as described below.
Axonal width measurement
To automate the process of axonal diameter measurement, we developed an ImageJ plugin (named 'Width Measurement') that measures pixel intensity across objects, finds the derivative to determine the steepest points, and then calculates the distance between these points . The plugin has previously been described in detail . In brief, the plugin uses the derivative of pixel intensity across the axon to find these steepest points and returns the distance between these points to calculate axonal width. To prepare images for analysis, images of axons acquired at 12-bit pixel depth were opened in ImageJ, converted to 32-bits, and straightened using the "Straighten"; plugin . The straightened images were then stretched 8× on the y-axis by interpolation using the ImageJ plugin TransformJ set to quintic B-spline . To remove high frequency noise, a Gaussian Blur filter with a radius 2 pixels was applied using the built-in ImageJ function. The 'Width Measurement' plugin was then run to determine axonal width at each pixel along the axon. The source code for the Plugin is available on request.
Growth cone area measurement
To determine the area of growth cones, the width and length of individual growth cones were measured from phase images using the line tool in ImageJ. These numbers were then multiplied to give growth cone area. While our goal was to simply determine if there is a change in growth cone size, we note that because growth cones are not perfectly square, the calculated numbers for growth cone area are an overestimate.
All animal studies were approved by the Michigan State University Institutional Animal Care and Use Committee.
Financial support was provided to PL, MO, and KM through startup funds from Michigan State University and a grant from the National Science Foundation Proposal Number 0951019 to K.E.M. This manuscript has been neither published nor submitted for publication elsewhere. There are no conflicts of interest with the material presented in this paper. Correspondence and requests for materials should be addressed to Kyle E. Miller, firstname.lastname@example.org.
All animal studies were approved by the Michigan State University Institutional Animal Care and Use Committee.
- Verdu E, Ceballos D, Vilches JJ, Navarro X: Influence of aging on peripheral nerve function and regeneration. J Peripher Nerv Syst. 2000, 5 (4): 191-208. 10.1046/j.1529-8027.2000.00026.x.View ArticlePubMedGoogle Scholar
- Pestronk A, Drachman DB, Griffin JW: Effects of aging on nerve sprouting and regeneration. Exp Neurol. 1980, 70 (1): 65-82. 10.1016/0014-4886(80)90006-0.View ArticlePubMedGoogle Scholar
- Campbell WW: Evaluation and management of peripheral nerve injury. Clin Neurophysiol. 2008, 119 (9): 1951-1965. 10.1016/j.clinph.2008.03.018.View ArticlePubMedGoogle Scholar
- Hoke A: Mechanisms of Disease: what factors limit the success of peripheral nerve regeneration in humans?. Nat Clin Pract Neurol. 2006, 2 (8): 448-454. 10.1038/ncpneuro0262.View ArticlePubMedGoogle Scholar
- DiGiovanni S, Faden AI, Yakovlev A, Duke-Cohan JS, Finn T, Thouin M, Knoblach S, De Biase A, Bregman BS, Hoffman EP: Neuronal plasticity after spinal cord injury: identification of a gene cluster driving neurite outgrowth. Faseb J. 2005, 19 (1): 153-154.Google Scholar
- Makwana M, Raivich G: Molecular mechanisms in successful peripheral regeneration. Febs J. 2005, 272 (11): 2628-2638. 10.1111/j.1742-4658.2005.04699.x.View ArticlePubMedGoogle Scholar
- Zou H, Ho C, Wong K, Tessier-Lavigne M: Axotomy-induced Smad1 activation promotes axonal growth in adult sensory neurons. J Neurosci. 2009, 29 (22): 7116-7123. 10.1523/JNEUROSCI.5397-08.2009.PubMed CentralView ArticlePubMedGoogle Scholar
- Smith DH: Stretch growth of integrated axon tracts: extremes and exploitations. Prog Neurobiol. 2009, 89 (3): 231-239. 10.1016/j.pneurobio.2009.07.006.PubMed CentralView ArticlePubMedGoogle Scholar
- Siechen S, Yang S, Chiba A, Saif T: Mechanical tension contributes to clustering of neurotransmitter vesicles at presynaptic terminals. Proc Natl Acad Sci USA. 2009, 106 (31): 12611-12616. 10.1073/pnas.0901867106.PubMed CentralView ArticlePubMedGoogle Scholar
- Franze K, Reichenbach A, Kas J: Biomechanics of the CNS. Mechanosensitivity of the Nervous System. Edited by: Kamkin A, Kiseleva I. 2009, Springer Netherlands, 2: 173-213. full_text.View ArticleGoogle Scholar
- O'Toole M, Lamoureux P, Miller KE: A physical model of axonal elongation: force, viscosity, and adhesions govern the mode of outgrowth. Biophys J. 2008, 94 (7): 2610-2620. 10.1529/biophysj.107.117424.PubMed CentralView ArticlePubMedGoogle Scholar
- Bernal R, Pullarkat PA, Melo F: Mechanical properties of axons. Phys Rev Lett. 2007, 99 (1): 018301-10.1103/PhysRevLett.99.018301.View ArticlePubMedGoogle Scholar
- Chan CE, Odde DJ: Traction dynamics of filopodia on compliant substrates. Science. 2008, 322 (5908): 1687-1691. 10.1126/science.1163595.View ArticlePubMedGoogle Scholar
- Bueno FR, Shah SB: Implications of tensile loading for the tissue engineering of nerves. Tissue Eng Part B Rev. 2008, 14 (3): 219-233. 10.1089/ten.teb.2008.0020.View ArticlePubMedGoogle Scholar
- Miller KE, Sheetz MP: Direct evidence for coherent low velocity axonal transport of mitochondria. J Cell Biol. 2006, 173 (3): 373-381. 10.1083/jcb.200510097.PubMed CentralView ArticlePubMedGoogle Scholar
- Lamoureux P, Heidemann SR, Martzke NR, Miller KE: Growth and elongation within and along the axon. Dev Neurobiol. 2010, 70 (3): 135-149.View ArticlePubMedGoogle Scholar
- Lindsay RM: Nerve growth factors (NGF, BDNF) enhance axonal regeneration but are not required for survival of adult sensory neurons. J Neurosci. 1988, 8 (7): 2394-2405.PubMedGoogle Scholar
- Smith DS, Skene JH: A transcription-dependent switch controls competence of adult neurons for distinct modes of axon growth. J Neurosci. 1997, 17 (2): 646-658.PubMedGoogle Scholar
- Tonge D, Chan K, Zhu N, Panjwani A, Arno M, Lynham S, Ward M, Snape A, Pizzey J: Enhancement of axonal regeneration by in vitro conditioning and its inhibition by cyclopentenone prostaglandins. J Cell Sci. 2008, 121 (Pt 15): 2565-2577. 10.1242/jcs.024943.View ArticlePubMedGoogle Scholar
- Hoffman PN, Cleveland DW: Neurofilament and tubulin expression recapitulates the developmental program during axonal regeneration: induction of a specific beta-tubulin isotype. Proc Natl Acad Sci USA. 1988, 85 (12): 4530-4533. 10.1073/pnas.85.12.4530.PubMed CentralView ArticlePubMedGoogle Scholar
- Oblinger MM, Argasinski A, Wong J, Kosik KS: Tau gene expression in rat sensory neurons during development and regeneration. J Neurosci. 1991, 11 (8): 2453-2459.PubMedGoogle Scholar
- Jones SL, Selzer ME, Gallo G: Developmental regulation of sensory axon regeneration in the absence of growth cones. J Neurobiol. 2006, 66 (14): 1630-1645. 10.1002/neu.20309.PubMed CentralView ArticlePubMedGoogle Scholar
- Lowery LA, Van Vactor D: The trip of the tip: understanding the growth cone machinery. Nat Rev Mol Cell Biol. 2009, 10 (5): 332-343. 10.1038/nrm2679.PubMed CentralView ArticlePubMedGoogle Scholar
- Conde C, Caceres A: Microtubule assembly, organization and dynamics in axons and dendrites. Nat Rev Neurosci. 2009, 10 (5): 319-332. 10.1038/nrn2631.View ArticlePubMedGoogle Scholar
- Muir D: The potentiation of peripheral nerve sheaths in regeneration and repair. Exp Neurol. 2010, 223 (1): 102-111. 10.1016/j.expneurol.2009.05.038.View ArticlePubMedGoogle Scholar
- Wallquist W, Patarroyo M, Thams S, Carlstedt T, Stark B, Cullheim S, Hammarberg H: Laminin chains in rat and human peripheral nerve: distribution and regulation during development and after axonal injury. J Comp Neurol. 2002, 454 (3): 284-293. 10.1002/cne.10434.View ArticlePubMedGoogle Scholar
- Carulli D, Laabs T, Geller HM, Fawcett JW: Chondroitin sulfate proteoglycans in neural development and regeneration. Curr Opin Neurobiol. 2005, 15 (1): 116-120. 10.1016/j.conb.2005.01.014.View ArticlePubMedGoogle Scholar
- Zuo J, Hernandez YJ, Muir D: Chondroitin sulfate proteoglycan with neurite-inhibiting activity is up-regulated following peripheral nerve injury. J Neurobiol. 1998, 34 (1): 41-54. 10.1002/(SICI)1097-4695(199801)34:1<41::AID-NEU4>3.0.CO;2-C.View ArticlePubMedGoogle Scholar
- Werner A, Willem M, Jones LL, Kreutzberg GW, Mayer U, Raivich G: Impaired axonal regeneration in alpha7 integrin-deficient mice. J Neurosci. 2000, 20 (5): 1822-1830.PubMedGoogle Scholar
- Condic ML: Adult neuronal regeneration induced by transgenic integrin expression. J Neurosci. 2001, 21 (13): 4782-4788.PubMedGoogle Scholar
- Vogelezang MG, Liu Z, Relvas JB, Raivich G, Scherer SS, ffrench-Constant C: Alpha4 integrin is expressed during peripheral nerve regeneration and enhances neurite outgrowth. J Neurosci. 2001, 21 (17): 6732-6744.PubMedGoogle Scholar
- Blackmore M, Letourneau PC: L1, beta1 integrin, and cadherins mediate axonal regeneration in the embryonic spinal cord. J Neurobiol. 2006, 66 (14): 1564-1583. 10.1002/neu.20311.View ArticlePubMedGoogle Scholar
- Heidemann SR, Wirtz D: Towards a regional approach to cell mechanics. Trends Cell Biol. 2004, 14 (4): 160-166. 10.1016/j.tcb.2004.02.003.View ArticlePubMedGoogle Scholar
- Vogelaar CF, Hoekman MF, Gispen WH, Burbach JP: Homeobox gene expression in adult dorsal root ganglia during sciatic nerve regeneration: is regeneration a recapitulation of development?. Eur J Pharmacol. 2003, 480 (1-3): 233-250. 10.1016/j.ejphar.2003.08.110.View ArticlePubMedGoogle Scholar
- Kaverina I, Krylyshkina O, Beningo K, Anderson K, Wang YL, Small JV: Tensile stress stimulates microtubule outgrowth in living cells. J Cell Sci. 2002, 115 (Pt 11): 2283-2291.PubMedGoogle Scholar
- Oblinger MM, Szumlas RA, Wong J, Liuzzi FJ: Changes in cytoskeletal gene expression affect the composition of regenerating axonal sprouts elaborated by dorsal root ganglion neurons in vivo. J Neurosci. 1989, 9 (8): 2645-2653.PubMedGoogle Scholar
- Yuan A, Sasaki T, Rao MV, Kumar A, Kanumuri V, Dunlop DS, Liem RK, Nixon RA: Neurofilaments form a highly stable stationary cytoskeleton after reaching a critical level in axons. J Neurosci. 2009, 29 (36): 11316-11329. 10.1523/JNEUROSCI.1942-09.2009.PubMed CentralView ArticlePubMedGoogle Scholar
- Kushkuley J, Chan WK, Lee S, Eyer J, Leterrier JF, Letournel F, Shea TB: Neurofilament cross-bridging competes with kinesin-dependent association of neurofilaments with microtubules. J Cell Sci. 2009, 122 (Pt 19): 3579-3586. 10.1242/jcs.051318.View ArticlePubMedGoogle Scholar
- Leterrier JF, Janmey PA, Eyer J: Microtubule-independent regulation of neurofilament interactions in vitro by neurofilament-bound ATPase activities. Biochem Biophys Res Commun. 2009, 384 (1): 37-42. 10.1016/j.bbrc.2009.04.045.PubMed CentralView ArticlePubMedGoogle Scholar
- Leterrier JF, Kas J, Hartwig J, Vegners R, Janmey PA: Mechanical effects of neurofilament cross-bridges. Modulation by phosphorylation, lipids, and interactions with F-actin. J Biol Chem. 1996, 271 (26): 15687-15694. 10.1074/jbc.271.26.15687.View ArticlePubMedGoogle Scholar
- Nunez J, Fischer I: Microtubule-associated proteins (MAPs) in the peripheral nervous system during development and regeneration. J Mol Neurosci. 1997, 8 (3): 207-222. 10.1007/BF02736834.View ArticlePubMedGoogle Scholar
- Riederer BM: Microtubule-associated protein 1B, a growth-associated and phosphorylated scaffold protein. Brain Res Bull. 2007, 71 (6): 541-558. 10.1016/j.brainresbull.2006.11.012.View ArticlePubMedGoogle Scholar
- Svensson M, Aldskogius H: The effect of axon injury on microtubule-associated proteins MAP2, 3 and 5 in the hypoglossal nucleus of the adult rat. J Neurocytol. 1992, 21 (3): 222-231. 10.1007/BF01194980.View ArticlePubMedGoogle Scholar
- Chambers CB, Muma NA: Tau mRNA isoforms following sciatic nerve axotomy with and without regeneration. Brain Res Mol Brain Res. 1997, 48 (1): 115-124. 10.1016/S0169-328X(97)00089-2.View ArticlePubMedGoogle Scholar
- Costigan M, Befort K, Karchewski L, Griffin RS, D'Urso D, Allchorne A, Sitarski J, Mannion JW, Pratt RE, Woolf CJ: Replicate high-density rat genome oligonucleotide microarrays reveal hundreds of regulated genes in the dorsal root ganglion after peripheral nerve injury. BMC Neurosci. 2002, 3: 16-10.1186/1471-2202-3-16.PubMed CentralView ArticlePubMedGoogle Scholar
- Mechaly I, Bourane S, Piquemal D, Al-Jumaily M, Venteo S, Puech S, Scamps F, Valmier J, Carroll P: Gene profiling during development and after a peripheral nerve traumatism reveals genes specifically induced by injury in dorsal root ganglia. Mol Cell Neurosci. 2006, 32 (3): 217-229. 10.1016/j.mcn.2006.04.004.View ArticlePubMedGoogle Scholar
- Raivich G, Makwana M: The making of successful axonal regeneration: genes, molecules and signal transduction pathways. Brain Res Rev. 2007, 53 (2): 287-311. 10.1016/j.brainresrev.2006.09.005.View ArticlePubMedGoogle Scholar
- Bonilla IE, Tanabe K, Strittmatter SM: Small proline-rich repeat protein 1A is expressed by axotomized neurons and promotes axonal outgrowth. J Neurosci. 2002, 22 (4): 1303-1315.PubMedGoogle Scholar
- Lindsay RM, Lockett C, Sternberg J, Winter J: Neuropeptide expression in cultures of adult sensory neurons: modulation of substance P and calcitonin gene-related peptide levels by nerve growth factor. Neuroscience. 1989, 33 (1): 53-65. 10.1016/0306-4522(89)90310-2.View ArticlePubMedGoogle Scholar
- Heidemann SR, Lamoureux P, Ngo K, Reynolds M, Buxbaum RE: Open-dish incubator for live cell imaging with an inverted microscope. Biotechniques. 2003, 35 (4): 708-714, 716.PubMedGoogle Scholar
- Dennerll TJ, Lamoureux P, Buxbaum RE, Heidemann SR: The cytomechanics of axonal elongation and retraction. J Cell Biol. 1989, 109 (6 Pt 1): 3073-3083. 10.1083/jcb.109.6.3073.View ArticlePubMedGoogle Scholar
- Chang S, Rodionov VI, Borisy GG, Popov SV: Transport and turnover of microtubules in frog neurons depend on the pattern of axonal growth. J Neurosci. 1998, 18 (3): 821-829.PubMedGoogle Scholar
- Kocsis E, Trus BL, Steer CJ, Bisher ME, Steven AC: Image averaging of flexible fibrous macromolecules: the clathrin triskelion has an elastic proximal segment. J Struct Biol. 1991, 107 (1): 6-14. 10.1016/1047-8477(91)90025-R.View ArticlePubMedGoogle Scholar
- Meijering EH, Niessen WJ, Viergever MA: Quantitative evaluation of convolution-based methods for medical image interpolation. Med Image Anal. 2001, 5 (2): 111-126. 10.1016/S1361-8415(00)00040-2.View ArticlePubMedGoogle Scholar