Auditory brainstem responses are resistant to pharmacological modulation in Sprague Dawley wild-type and Neurexin1α knockout rats

Table 1 Shows the different animal cohorts used according to each pharmacological treatment and anesthesia type in both wild-type (WT) and Nrxn1α KO Sprague Dawley rats (KO). N/A: pharmacological treatment is not applicable

Animal Cohort	Anesthesia type	Pharmacology	Animal numbers	Animal age
Group A	Isoflurane	Nicotine	WT (N = 14), KO (N = 14)	20 weeks old
		Bitopertin	WT (N = 14), KO (N = 11)
		Baclofen	WT (N = 14), KO (N = 13)
	Ketamine/xylazine	N/A	WT (N = 9), KO (N = 9)	25 weeks old
Group B	Medetomidine	Diazepam	WT (N = 12), KO (N = 12)	25 weeks old
		Bitopertin
		Retigabine
		N/A		27 weeks old
	Isoflurane	N/A		27 weeks old
Group C	Isoflurane	Diazepam	WT (N = 14), KO (N = 14)	20 weeks old
Group C	Isoflurane	Gaboxadol	WT (N = 14), KO (N = 14)	20 weeks old
Group D	Isoflurane	Gaboxadol	WT (N = 18), KO (N = 16)	20 weeks old
Group D	Isoflurane	Retigabine	WT (N = 18), KO (N = 16)	20 weeks old

All procedures were approved by the Federal Food Safety and Veterinary Office of Switzerland (Basel) and conducted in adherence to the Swiss federal ordinance on animal protection and welfare, as well as according to the rules of the Association for Assessment and Accreditation of Laboratory Animal Care International and the ARRIVE guidelines [34]

ISSN: 1471-2202