Fig. 4

Auditory brainstem responses post pharmacological treatment in WT Sprague Dawley rats under medetomidine anesthesia. ABR waveforms across different stimulus intensities (90, 70, 50 dB) post intraperitoneal injection with diazepam (3 mg/kg) in magenta; (N = 12), bitopertin (10 mg/kg) in purple; (N = 12), retigabine (3 mg/kg) in red; (N = 12), or vehicle solution in black (0.9% saline + 0.3% Tween). Within each experimental block, dosing was counterbalanced, and applied 15 min prior to the ABR recordings for all compounds, except for in bitopertin (60 min pre-treatment time). Data displayed as mean ± SEM, was tested with unpaired CBPT. No robust significant differences were found between genotypes across anesthesia methods. Grey bars above the graphs indicate clusters of significant differences before CBPT-based correction for multiple comparisons, i.e., indicating statistical trends