Fig. 8

NGN2-AD2 co-culture: Single-cell calcium activity. A Exemplary image of DIV28 WT NGN2/AD2 co-cultures. Neurons were stained with Calbryte^™ 590 AM for analysis of intracellular calcium dynamics. NGN2 (eGFP-Calbryte-double positive) and AD2 neurons (eGFP negative) were examined, separately. Scale bars: 50 µm. B Exemplary calcium traces of WT and DISC1 ± NGN2 and AD2 neurons. Mean gray values of individual cell somata are plotted over a recording period of 3 min (583 frames). C–F Somatic calcium trace properties of NGN2 neurons in co-cultures. Peak amplitude = ΔF/F0), frequency (Hz), full width half maximum = FWHM, area under the curve = AUC. Data were retrieved from three independent experiments, with a minimum of three wells recorded per experiment and group (C: WT n = 15, DISC1 ± n = 16, WT = 1 ± 0.12, DISC1 ±  = 1.44 ± 0.19, Mann Whitney test, two-tailed, p = 0.1195 D: WT n = 15, DISC1 ± n = 16, WT = 0.02 ± 0.003, DISC1 ±  = 0.03 ± 0.003, Mann Whitney test, two-tailed, p = 0.1122 E: WT n = 15, DISC1 ± n = 16, WT = 1 ± 0.07, DISC1 ±  = 0.9 ± 0.12, Mann Whitney test, two-tailed, p = 0.0933 F: WT n = 15, DISC1 ± n = 16, WT = 1 ± 0.19, DISC1 ±  = 1.23 ± 0.21, Mann Whitney test, two-tailed, p = 0.5196). G-J Somatic calcium traces retrieved from AD2 neurons in co-cultures. Data were obtained from three independent experiments, with four wells recorded per experiment and condition (G: WT n = 16, DISC1 ± n = 16, WT = 1 ± 0.07, DISC1 ±  = 1.25 ± 0.09, unpaired t-test, two-tailed, p = 0.0362 H: WT n = 16, DISC1 ± n = 16, WT = 0.029 ± 0.003, DISC1 ±  = 0.026 ± 0.001, unpaired t-test, two-tailed, p = 0.4002 I: WT n = 16, DISC1 ± n = 16, WT = 1 ± 0.04, DISC1 ±  = 0.98 ± 0.05, unpaired t-test, two-tailed, p = 0.8103 J: WT n = 16, DISC1 ± n = 16, WT = 1 ± 0.11, DISC1 ±  = 1.22 ± 0.14, unpaired t-test, two-tailed, p = 0.2189)