Fig. 6

NGN2-AD2 co-culture: Synaptic marker densities in DISC1 ± AD2 neurons. A-A’ Immunostaining for excitatory synapse marker densities on MAP2-positive, eGFP-negative WT AD2 neurons (A) and DISC1 ± AD neurons (A’). Exemplary co-localizations of pre- and postsynaptic markers are indicated by arrows. Scale bars: 20 µm. B-B’ Immunostaining for inhibitory synapse marker densities on MAP2-positive, eGFP-negative WT AD2 neurons (B) and DISC1 ± AD2 neurons (B’). Exemplary co-localizations of pre- and postsynaptic markers are indicated by arrows. Scale bars: 20 µm. C–F Quantification of glutamatergic and GABAergic synapse marker densities on MAP2-positive, eGFP-negative dendrites in WT and DISC1 ± AD2 neurons. Data were obtained from four independent differentiations. Error bars: s.e.m. (C) presynaptic VGLUT1, WT n = 7, DISC1 ± n = 8, WT = 1 ± 0.09, DISC1 ±  = 1.07 ± 0.16, Mann–Whitney U test, two-tailed, p = 0.9551 (D) postsynaptic PSD95, WT n = 7, DISC1 ± n = 8, WT = 1 ± 0.09, DISC1 ±  = 1.77 ± 0.44, Mann–Whitney U test, two-tailed, p = 0.2319. E presynaptic vGAT, WT n = 8, DISC1 ± n = 8, WT = 1 ± 0.11, DISC1 ±  = 4.13 ± 0.78, Mann–Whitney U test, two-tailed, p = 0.0002. F postsynaptic gephyrin WT n = 8, DISC1 ± n = 8, WT = 1 ± 0.18, DISC1 ±  = 1.82 ± 0.61, Mann–Whitney U test, two-tailed, p = 0.5737