Fig. 5

NGN2-AD2 co-culture: Synaptic marker densities in DISC1 ± NGN2 neurons. A-A’ Immunostaining for excitatory synapse marker densities on eGFP-positive WT NGN2 neurons (A) and DISC1 ± NGN2 neurons (A’). Exemplary co-localizations of pre- and postsynaptic markers are indicated by arrows. Scale bars: 20 µm. B-B’ Immunostaining for inhibitory synapse markers on eGFP-positive WT NGN2 neurons (B) and DISC1 ± NGN2 neurons (B’). Exemplary co-localizations of pre- and postsynaptic markers are indicated by arrows. Scale bars: 20 µm. C–F Quantification of glutamatergic and GABAergic synapse marker densities on MAP2/eGFP double-positive dendrites in WT and DISC1 ± NGN2 neurons. Data were obtained from four independent differentiations. Error bars: s.e.m. (C) presynaptic VGLUT1, WT n = 7, DISC1 ± n = 8, WT = 1 ± 0.07, DISC1 ±  = 0.84 ± 0.16, Mann-Whitney U test, two-tailed, p = 0.3969 (D) postsynaptic PSD95, WT n = 7, DISC1 ± n = 8, WT = 1 ± 0.12, DISC1 ±  = 1.11 ± 0.23, Mann-Whitney U test, two-tailed, p = 0.8665 (E) presynaptic vGAT, WT n = 8, DISC1 ± n = 8, WT = 1 ± 0.04, DISC1 ±  = 2.38 ± 0.08, Mann-Whitney U test, two-tailed, p = 0.0002. F postsynaptic gephyrin, WT n = 8, DISC1 ± n = 8, WT = 1 ± 0.19, DISC1 ±  = 1.39 ± 0.29, Mann-Whitney U test, two-tailed, p = 0.3823.