Fig. 4

Common and divergent protein profiles in DISC1 ± NGN2 and AD2 neurons. A, B Bar chart representations of selected analytes measured in NGN2 (A) and AD2 (B) neurons. Data were normalized to WT signals within individual replicates, data points from the same replicate were connected. (DVL2 Segment polarity protein dishevelled homolog DVL-2, GSK3 Glycogen synthase kinase-3, PSD95 Postsynaptic density protein 95, CAMKIV Calcium/calmodulin-dependent protein kinase type IV, AKT1 RAC-alpha serine/threonine-protein kinase, PDK1 phosphoinositide-dependent protein kinase 1, PLCγ1 Phospholipase C gamma 1, PAK1/2/3 Serine/threonine-protein kinase PAK 1/2/3, STAT3 Signal transducer and activator of transcription 3, ILK1 Integrin-linked protein kinase 1, MAPK1 mitogen-activated protein kinase 1). Error bars: s.e.m. (NGN2: β-catenin WT = 1 ± 0.09, DISC1 ±  = 0.8 ± 0.1, DVL2 WT = 1 ± 0.1, DISC1 ±  = 0.6 ± 0.07, STAT3-pY705 WT = 1 ± 0.16, DISC1 ±  = 0.55 ± 0.12, Cyclin D2 WT = 1 ± 0.14, DISC1 ±  = 1.39 ± 0.26, GSK3β WT = 1 ± 0.06, DISC1 ±  = 0.89 ± 0.02, casein kinase 1ε WT = 1 ± 0.15, DISC1 ±  = 1.58 ± 0.16, AKT1 WT = 1 \(\pm\) 0.1, DISC1 ±  = 0.79 ± 0.06, PDK1 WT = 1 ± 0.12, DISC1 ±  = 0.7 ± 0.06, PDK1-pS241 WT = 1 ± 0.04, DISC1 ±  = 0.83 \(\pm\) 0.03, GSK3α WT = 1 ± 0.14, DISC1 ±  = 0.92 \(\pm\) 0.04, PLCγ1–pS1248 WT = 1 ± 0. 19, DISC1 ±  = 1.3 ± 0.21, PAK1/2/3 WT = 1 ± 0.06, DISC1 ±  = 0.88 ± 0.04, STAT3-pS272 WT = 1 ± 0.07, DISC1 ±  = 0.69 ± 0.11; AD2: β-catenin WT = 1 ± 0.05, DISC1 ±  = 0.85 ± 0.02, DVL2 WT = 1 ± 0.1, DISC1 ±  = 0.77 ± 0.11, STAT3-pY705 WT = 1 ± 0.35, DISC1 ±  = 0.51 ± 0.07, Cyclin D2 WT = 1 ± 0.12, DISC1 ±  = 1.45 ± 0.24, casein kinase 1α WT = 1 ± 0.07, DISC1 ±  = 0.88 ± 0.02, PAK1/2-pS144/141 WT = 1 ± 0.1, DISC1 ±  = 1.35 ± 0.11, ILK1-pS343 WT = 1 ± 0.07, DISC1 ±  = 0.83 ± 0.09, MAPK1 WT = 1 ± 0.07, DISC1 ±  = 0.77 ± 0.12)