Fig. 3

Inhibitory synapse density in DISC1 ± AD2 neurons. A Scheme of AD2 neuron differentiation. iPSC were first differentiated into NPC and subsequently transduced with doxycycline-inducible ASCL1 and DLX2. B Fluorescence microscopy for microtubule-associated protein 2 (MAP2) and GABA immunostaining in AD2 neurons. Scale bars: 50 µm. C–C’ Expression of MAP2, presynaptic marker vesicular GABA transporter (vGAT) and postsynaptic marker Gephyrin in WT (C) or DISC1 ± (C’) AD2 neurons. Scale bars: 20 µm. D-F Quantification of GABAergic synapse marker densities in DISC1 ± AD2 neurons; WT n = 6, DISC1 ± n = 6. Data were obtained from three independent differentiations. D Inhibitory synapse densities (co-localization of vGAT + gephyrin): WT = 1 ± 0.18, DISC1 ±  = 1.45 ± 0.5, Mann–Whitney U test, two-tailed, p = 0.8182. E Presynaptic vGAT cluster densities: WT = 1 ± 0.07, DISC1 ±  = 0.98 ± 0.28, Mann–Whitney U test, two-tailed, p = 0.3939. F Postsynaptic gephyrin cluster densities: WT = 1 ± 0.22, DISC1 ±  = 0.99 ± 0.35, Mann–Whitney U test, two-tailed, p = 0.9372. Error bars: s.e.m