Fig. 2

Excitatory synapse density in DISC1 ± NGN2 neurons. A Scheme of NGN2 neuron differentiation. iPSC were first differentiated into NPC and subsequently transduced with doxycycline-inducible hNGN2-eGFP. B Fluorescence microscopy for microtubule-associated protein 2 (MAP2) immunostaining and detection of eGFP expression in NGN2 neurons. Scale bars: 20 µm. C–C’ Expression of MAP2, presynaptic marker vesicular glutamate transporter 1 (vGLUT1) and postsynaptic marker postsynaptic density protein 95 (PSD95) in WT (C) or DISC1 ± (C’) NGN2 neurons. Scale bars: 20 µm. D–F Quantification of excitatory synapse marker densities in DISC1 ± NGN2 neurons; WT n = 10, DISC1 ± n = 10. Data were collected from five independent differentiations. D Excitatory synapse densities defined as apposition of presynaptic vGLUT1 and postsynaptic PSD95 clusters: WT = 1 ± 0.05, DISC1 ±  = 0.65 ± 0.08, Mann–Whitney U test, two-tailed, p = 0.0039. E Presynaptic vGLUT1 cluster densities: WT = 1 ± 0.02, DISC1 ±  = 0.85 ± 0.09, Mann–Whitney U test, two-tailed, p = 0.1431. F Postsynaptic PSD95 cluster densities: WT = 1 ± 0.06, DISC1 ±  = 1.02 \(\pm\) 0.2, Mann–Whitney U test, two-tailed, p = 0.7959). Error bars: s.e.m