Fig. 1

Neurite outgrowth of differentiating DISC1 ± NPCs. A DISC1 immunocytochemistry of WT and DISC1 ± iPSC and NPC. iPSC are OCT4 positive (Scale bars: 100 µm), and subpopulations of NPC are PAX6 positive (Scale bars: 50 µm). B, C Quantification of DISC1 fluorescence intensity of WT and DISC1 ± iPSC (WT = 1 ± 0.07, DISC1 ±  = 0.7 ± 0.07) and NPC (WT = 1 ± 0.07, DISC1 ±  = 0.7 ± 0.08). Data points represent averaged fluorescence intensities of multiple cells within a single microscopic field. Data were obtained from three independent experiments. iPSC: WT n = 23, DISC1 ± n = 25, Mann–Whitney U test, two-tailed, p = 0.002. NPC: WT n = 24, DISC1 ± n = 23, Mann–Whitney U test, two-tailed, p = 0.0008. D Neurite outgrowth assay of differentiating NPC. Neurite length per cell body area was measured every 4 h over a period of 16 h and was significantly reduced in DISC1 ± cells at t = 12 h and t = 16 h (0 h: WT = 36.6 ± 4.2, DISC1 ±  = 29 ± 2.9; 4h: WT = 42.4 ± 4.4, DISC1 ±  = 32.2 ± 3.3; 8h: WT = 47.7 ± 3.9, DISC1 ±  = 35.4 ± 3.9; 12 h: WT = 48.3 ± 3.5, DISC1 ±  = 34.5 ± 3.7; 16h: WT = 47.6 ± 3.2, DISC1 ±  = 33.9 ± 3.7). Data were obtained from 4 independent experiments with at least 3 wells (4 images each) imaged per condition and replicate. WT n = 24, DISC1 ± n = 24 (for each timepoint). (2way ANOVA with Šídák's multiple comparisons test, t = 12 h p = 0.0423, t = 16 h p = 0.0463). Error bars: s.e.m