Fig. 3

BV2 cells do not recapitulate Tmem119-expression patterns of primary microglia. (A) BV2 cells were treated with 5 μg/mL LPS for 24 h and collected for experiments. (B) Flow cytometry histograms of Tmem119-PE/Cy7 on BV2 cells in control and LPS groups. (C) Quantification of BV2 Tmem119-PE/Cy7 MFI in control and LPS groups. P values are the result of one-way ANOVA with Tukey HSD. N = 3 replicates of BV2 cells. (D) RT-qPCR of Tmem119 expression in BV2 cells in control and LPS groups. P value is the result of unpaired two-tailed T-test. N = 3 replicates of BV2 cells. (E) Comparison of Tmem119 expression in BV2 cells and primary microglia. (F) Flow cytometry histogram of Tmem119-PE/Cy7 in BV2 cells and primary microglia. (G) Quantification of Tmem119-PE/Cy7 MFI in BV2 cells and primary microglia. P value is the result of unpaired two-tailed T-test. N = 3 replicates of BV2 cells and primary microglia from 3 mice. (H) Relative expression of Tmem119 detected by RT-qPCR in BV2 cells and primary microglia. P value is the result of unpaired two-tailed T-test. N = 3 replicates of BV2 cells and primary microglia from 3 mice