Fig. 2
From: Tmem119 expression is downregulated in a subset of brain metastasis-associated microglia
Changes in microglia Tmem119 expression after LPS treatment. (A) Tmem119-eGFP mice were treated with 5 mg/kg LPS for 24 h prior to analysis. (B) Representative flow cytometry dot plot and histogram of Tmem119-eGFP signal in control and LPS groups. (C) Quantification of Tmem119-eGFP MFI. P value is the result of unpaired two-tailed T-test. N = 4 mice per group. (D) RT-qPCR results of Tmem119 expression in microglia sorted from brains of control and LPS groups. P value is the result of unpaired two-tailed T-test. N = 3 mice per group. (E) Representative flow cytometry dot plot and histogram of Tmem119-eGFP in microglia from various brain regions in control and LPS groups. (F) Quantification of microglia Tmem119-eGFP MFI from various brain regions in control and LPS groups. P values are the result of unpaired two-tailed T-tests. N = 4 control and 5 LPS-treated mice. (G) Quantification of GFPlow microglia population in control and LPS groups from various brain regions. P values are the result of unpaired two-tailed T-tests. N = 4 control and 5 LPS-treated mice