Fig. 1
From: Tmem119 expression is downregulated in a subset of brain metastasis-associated microglia
Basal Tmem119 expression in primary murine microglia (MG). (A) Gating strategy for primary microglia (CD45int CD11bpos) from mouse brains. (B) Representative flow cytometry histograms of Tmem119-PE/CY7 compared to isotype controls. (C) Quantification of Tmem119-PE/CY7 MFI. P value is the result of unpaired two-tailed T-test. Data are presented as mean ± S.D. N = 3 mice. (D) Microglia from cortex (CX), midbrain (MB), and cerebellum (CBM) of naïve Tmem119-eGFP transgenic mice were compared for Tmem119 expression levels. (E) Flow cytometry histograms of Tmem119-eGFP from microglia in various brain regions. (F) Quantification of Tmem119-eGFP MFI. P values are results of One-way ANOVA with Tukey HSD. Data are presented as mean ± S.D. N = 4 mice. (G) Representative immunofluorescence microscopy images of Tmem119-eGFP microglia in different brain regions. Scale bar = 80 μm. Insets (yellow borders): Enlarged area showing single microglia. (H) Quantification of GFP-staining intensity in microglia from different brain regions. P values are the result of one-way ANOVA with Tukey HSD. N = 285 CX microglia, N = 162 MB microglia, and N = 52 CBM microglia from 3 different sections per region