Fig. 2

Thresholds of ABR and DPOAEs (mean ± standard deviation) and representative images of the rat cochlea (magnification ×20) showed OHCs loss at 14 days post-noise. a In vivo experiment procedures. b and f At 1 h post-noise. c and g At 3 days post-noise. d and h At 7 days post-noise. e and i At 14 days post-noise. Data were analysed using a two-way analysis of variance (ANOVA) followed by Tukey post-hoc test (n = 8 per group). * p < 0.05. j Phalloidin labelling showed OHCs survival in the cochlea after SCGx and noise. k Quantification of phalloidin-positive OHCs. Data were analysed with Dunnett’s multiple comparisons (repeated measures). Scale bar = 50 μm. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001