Fig. 5
Lysosomal Genes. A There was a significant main effect for treatment (F1,31 = 6.9, p = 0.01), CR increased TFE3 compared to no treatment, in Chow mice only. B There were no significant effects on TFE3 in males. C There was a significant main effect for treatment (F1,31 = 4.6, p = 0.04), CR increased FNIP2 in Chow females only. D There was a strong trending interaction (F1,33 = 3.3, p = 0.08), CR reduced FNIP2 transcripts in Obese males only. E There was a strong trending interaction (F1,29 = 3.6, p = 0.07) where higher levels of FLCN in Obese females were reduced by CR. F There were no significant effects of diet or treatment on FLCN levels in male mice. G There were no significant effects of diet or treatment on LAMP1 in female mice. H There were no significant effects of diet or treatment on LAMP1 levels in male mice. I There were no effects of diet or treatment on CTSB in female mice. J There were no effects of diet or treatment in males on CTSB. K There were no effects of diet or treatment in females on CTSD. L There were no significant effects of diet or treatment on CTSD in male mice. α indicates significant effect due to diet (Control vs. Obese), β indicates significant effect due to treatment (No treatment vs. CR), ^ indicates strong trend (0.09 ≥ p ≥ 0.05) in ANOVA results with significant post hoc test. All significance indicators represent a p-value of less than 0.05 unless otherwise indicated