Fig. 4

Autophagy Genes. A There was a strong trend for a treatment main effect (F_1,31 = 4.1, p = 0.05) where CR reduced Atg12 transcripts, regardless of diet. B There were significant main effects of both diet (F_1,33 = 12.7, p = 0.001) and treatment (F_2,33 = 16.3, p = 0.0003, whereby Obesity increased Atg12 transcripts across treatment groups, but CR reduced Atg12 in both Chow and Obese mice. C There were no effects of diet or treatment on Atg5 transcripts in female mice. D There were no effects of diet or treatment on Atg5 transcripts in male mice. E There were no effects of diet or treatment on Atg7 transcripts in female mice. F There was s significant main effect of treatment (F_1,33 = 7.5, p = 0.01), CR increased Atg7, particularly in Chow males. G There were no effects of diet or treatment on Becn1 in female mice. H There were no effects of diet or treatment on Beclin1 in male mice. I There were no effects of diet or treatment on SQSTM1 in female mice. J There were no effects of diet or treatment on SQSTM1 in males. K There were no effects of diet or treatment in females on Slc38a9. L There was a significant main effect of diet (F_1,33 = 8.1, p = 0.008), Obesity elevated Slc38a9 levels, particularly in CR males. α indicates significant effect due to diet (Control vs. Obese), β indicates significant effect due to treatment (No treatment vs. CR), ^ indicates strong trend (0.09 ≥ p ≥ 0.05) in ANOVA results with significant post hoc test. All significance indicators represent a p-value of less than 0.05 unless otherwise indicated