Fig. 3
Signaling Pathways. A There were no effects of diet or treatment in female mice on pAkt expression. B There was a significant interaction (F1,33 = 4.8, p = 0.04) in males whereby Obesity increased pAkt levels compared to Control, while CR reduced pAkt levels in Obese to levels commensurate with Control mice. C There was a significant main effect of diet (F1,30 = 6.7, p = 0.01), pAMPK levels were elevated in Obese females, significantly so in the CR treatment group. D There were no effects of either diet or treatment on pAMPK levels in males E. There was a strong trend (F1,29 = 3.5, p = 0.07) in females; within CR treatment Obese females had increased levels of pRPS6 compared to Control females. F There was a significant main effect of treatment (F1,32 = 16.1, p = 0.0003) in male pRPS6 expression. CR reduced pRPS6 regardless of diet. G There were no effects of diet or treatment in pULK in female mice. H There were no effects of diet or treatment in pULK levels in male mice. α indicates significant effect due to diet (Control vs. Obese), β indicates significant effect due to treatment (No treatment vs. CR), ^ indicates strong trend (0.09 ≥ p ≥ 0.05) in ANOVA results with significant post hoc test. All significance indicators represent a p-value of less than 0.05 unless otherwise indicated