Fig. 2
Watermaze Learning. A and B Learning across four days of training was expressed as area under the curve; the higher the value the poorer the learning. A There was a significant interaction for female learning (F1,30 = 18.9, p = 0.0001). Obesity produced learning deficits, but CR improved learning in Obese, but impaired learning in Control females. B There were no significant effects of diet or treatment in male learning behaviors. C There was a significant interaction (F1,30 = 6.3, p = 0.02) in platform crossings, Obese mice made fewer crossings, while CR reduced crossings for Control females. D There were no significant effects of diet or treatment in male platform crossings. E There was a trending interaction (F1,30 = 3.8, p = 0.06) and main effect of diet (F1,30 = 3.3, p = 0.08), significant post hoc tests confirm that Obesity alone produced increased latencies to finding the platform location. F There was a significant interaction (F1,34 = 4.7, p = 0.04) where CR in Chow males only reduced latencies to find the platform location. G There were no effects of diet or treatment in females on time in D quadrant (where the platform was located). H There were no effects of diet or treatment in male mice on time in D quadrant (where the platform was located). α indicates sig. difference due to diet (Control vs. Obese), β indicates sig. difference due to treatment (No treatment vs CR), δ indicates sig different from all other groups, ^ indicates strong trend (0.09 ≥ p ≥ 0.05) in ANOVA results with significant post hoc test. All significance indicators represent a p-value of less than 0.05 unless otherwise indicated