Table 1 Primer sequences used for real-time RT-PCR
Gene | Orientation | Sequence (5′ → 3′) |
|---|---|---|
SWS1 | Forward | TTCTCCAAGAGCTCCTGCGTGTACAA |
Reverse | TTAAGAGGCCGTGGACACCTCCG | |
SWS2a | Forward | TCAAAGGCCTCCACTGTGTACAATCC |
Reverse | CTAAGCTGGTCCGACTTTAGAGACTTC | |
SWS2b | Forward | CCACAGTCTACAACCCCTTCATTTATGTC |
Reverse | TTAGGAAGGGCCGACTTTTGAGACTTC | |
RH2a | Forward | AAAGAGCTCAGCCCTGTTCAATCCTATC |
Reverse | CAAGCAGCAGTAGAGACTTCTGTCTTGC | |
RH2b & RH2c | Forward | AAGAGCTCAGCATTGTACAATGCTGTTATCTA |
Reverse | TTAAGCTGCAGTTGAGACTTCTGTCTTGC | |
LWSa & LWSb | Forward | TTTGCAAAGAGCGCCACAATCTACAACC |
Reverse | TATGCAGGAGCCACAGAGGAGACC | |
Actb | Forward | AGCCCTGGCCCCATCCACCA |
Reverse | GAGGGGCCAGACTCATCGTACTC |