Fig. 6
Spectral sensitivity of the sws1/sws2/lws triple mutant. a Spectra of the LED light used for the OMR test in (b–f). The peak wavelengths should be 365, 450, 530, 630, and 730 nm according to the manufacturer; however, the data measured by the Sun Spectroradiometer S-2440 instrument (Soma Optics) showed that they were 367, 447, 521, 641, and 736 nm. b OMR under UV light (λ = 365 nm). The wild-type (n = 6; black) and triple-mutant (n = 6; green) fish were tested under seven PFD values of 0.0, 6.8 × 10–3, 7.0 × 10–2, 1.0 × 10–1, 1.4 × 10–1, 3.6 × 10–1, and 8.4 μmol/m2/s (as measured by the QTM-101 quantameter; Monotech). See Fig. 2c for details. Data of the replaced fish (see Results) were shown by cross marks (instead of dots). The results obtained at 8.4 μmol/m2/s (9.1–16.4 and 14.0–19.7 rounds [95% confident intervals] in the WT and triple mutant, respectively) were omitted from the graph. c OMR under blue light (λ = 450 nm) tested at 8.8 × 10–5, 3.3 × 10–3, 8.8 × 10–3, 4.1 × 10–2, and 9.0 × 10–2 μmol/m2/s. d OMR under green light (λ = 530 nm) tested at 4.2 × 10–4, 1.8 × 10–3, 2.1 × 10–3, 7.8 × 10–3, or 1.7 × 10–2 μmol/m2/s. e OMR under red light (λ = 630 nm) tested at 5.3 × 10–3, 2.0 × 10–1, 3.6 × 10–1, 4.7 × 10–1, or 6.4 × 10–1 μmol/m2/s. f OMR under near-IR light (λ = 730 nm) tested at 6.7 × 10–1, 1.5 × 10+1, 3.6 × 10+1, 5.0 × 10+1, or 6.4 × 10+1 μmol/m2/s