Fig. 2
Behavioral UV sensitivity of the sws1 or sws1/sws2 double mutants. a Establishment of the sws1/sws2 double mutants. Double heterozygotes for the sws1−10 and sws2+1a+14b mutations were intercrossed, and the offspring were raised under identical conditions until maturation; their genotypes (top) and phenotypes (bottom) are summarized in the tables. No significant difference was detected between the observed and expected ratios. b Normalized spectra of the UV light used for the experiments in c (blue) and d (violet). An IR spectrum of the IR camera for video recording is also shown (dark red). These spectra were measured at every 1 nm using a Sun Spectroradiometer S-2440 instrument (Soma Optics). c OMR under UV light (λ = 365 nm). Eight intensities of 0.0, 0.21, 0.27, 0.47, 1.4, 24, 77, and 130 μmol/m2/s (as measured by the Sun Spectroradiometer S-2440) were applied. The graphs of the WT (black), sws1 mutant (purple), and sws1/sws2 double mutant (blue) (n = 8 each) are horizontally shifted for viewing purposes. Each dot represents a result of one fish, and the results of the same fish at different intensities are connected by straight lines. The closed circles and vertical bars indicate the mean and the 95% confidence intervals of the mean, respectively. The horizontal orange line at the OMR of 20 rounds indicates that the fish perfectly followed the rotating stripes (10 rpm × 2 min). d OMR under UV light at a shorter wavelength (λ = 350 nm). We compared the WT and the sws1/sws2 double mutant (n = 8 each) at five intensities of 0.0, 5.1, 13, 20, and 25 μmol/m2/s. See (c) for details