Fig. 6

CaN regulated JEV-induced autophagy impairment (A) SK-N-SH cells were treated with FK506 (10 µM) for 1 h, and then infected with JEV. The protein levels of SQSTM1/p62 and LC3B were examined. (B) Bar graph representing the average SQSTM1/p62 protein levels. Values represent the mean ± SEM (n = 5). ***p < 0.001 vs. JEV. (C) After infection with JEV, the cells were incubated with GFP-LC3B lentivirus at an MOI of 30 for more than 18 h. Cell nuclei were stained with DAPI (blue) and analyzed by confocal microscopy. (D) Autophagy flux was examined by TEM. Arrowheads designate autophagosomes and arrow designate autolysosomes