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Fig. 3 | BMC Neuroscience

Fig. 3

From: Melatonin inhibits Japanese encephalitis virus replication and neurotoxicity via calcineurin-autophagy pathways

Fig. 3

Melatonin inhibited JEV-mediated neuroinflammatory response SK-N-SH cells were subjected to infection and melatonin treatment as described in Fig. 2. The mRNA (A, B, E, F) and protein (C, D, G, H) levels of TNF-α and IL-6 were measured via ELISA and quantitative real-time PCR, respectively. (A, B, E, F) The expression levels of TNF-α and IL-6 cytokines were evaluated by normalizing to those of GAPDH; the graph indicates the relative quantification of gene expression normalized to control samples. (C, D, G, H) The data show the mean concentrations (pg/ml) of TNF- α and IL-6 secreted in the supernatant. Values represent the mean ± SEM (n = 10). **p < 0.01, ***p < 0.001 vs. control (A, B, C, D) or JEV (E, F, G, H)

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BMC Neuroscience

ISSN: 1471-2202

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