Fig. 1
From: ANDA: an open-source tool for automated image analysis of in vitro neuronal cells
A flowchart of ANDA. Raw phase contrast images are segmented prior to identification and analysis of cell bodies or neurites. The mapping of cell bodies and neurites yield results of their own, or their outlines can be used to identify neurite attachment points. This is done by applying Sobel edge detection on the neurite outlines, followed by colour inversion of cell body outlines and neurite edges. Thereafter, the overlap between cell bodies and neurites are determined by image multiplication. These overlaps are quantified as neurite attachment points. The data is summarized in different csv files