Fig. 3

Recording regions and amperometric calculations. (A) Anatomical regions of interest (ROI) in the rodent brain were the hippocampus (blue), thalamus (green), and cortex (yellow). Image modified from Paxinos and Watson (2007). * represents the tip of the MEA. (B) Representative peak, showing glutamate concentration (µM) as a function of time in seconds in response to local applications of 100 µM glutamate. Amperometric calculations used in the analysis (peak amplitude, uptake rate, and T₈₀) are shown