Fig. 3

iPSC-NPC derived astrocytes do not hinder T cell proliferation. A, B Fluorescence images showing representative stainings for GFAP, nestin, S100β and vimentin in mouse (A) or human (B) iAstrocytes and percentage of cells positive for astrocyte markers (right panels). DAPI was used for nuclear staining. (C–F) CFSE levels in mouse ConA-activated CD3 + T lymphocytes cultured without (black lines) or with (red line) mouse iAstrocytes differentiated for 15 days (C–D) or 60 days (E–F). Graphs in D and F report the percentage of T cells which underwent 0, 1 or > 2 cell divisions. (G–J) CFSE levels in mouse ConA-activated CD3 + T lymphocytes cultured without (black lines) or with (red line) human iAstrocytes differentiated for 15 days (G, H) or 60 days (I–J). In C, E, G and I dotted lines represent mean CFSE levels in unstimulated cells (ns). In C–J representative data of one out of 3–7 independent experiments are shown and reported as mean ± SD. In A and B graphs report data from two iAstrocyte cell lines and bars represent SEM. Statistical analysis was performed using T test. *p-value < 0.05, ***p-value < 0.001