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Fig. 4 | BMC Neuroscience

Fig. 4

From: Hydrogen alleviated cognitive impairment and blood‒brain barrier damage in sepsis-associated encephalopathy by regulating ABC efflux transporters in a PPARα-dependent manner

Fig. 4

Treatment with 2% H2 related to PPARα mitigated the histopathologic changes and prevented neuronal apoptosis in septic mouse brain tissues. (A) and (C) Regular morphology of the pyramidal neurons in the Sham group was observed compared to the other groups. Many damaged neurons (marked with arrow), in which the Nissl body was decreasing or dissolving, were observed in the CLP and CLP + H2 + GW6471 groups. Neurons were significantly improved in the CLP + H2 group vs. the CLP and CLP + H2 + GW6471 groups. Scale bar = 100 μm. (B) Brain samples were harvested to measure TUNEL staining at 24 h after sham or CLP operation, and the TUNEL-positive cells per high-power field were counted (D). Apoptotic cells were stained green in the nuclei (marked with an arrow). DNA in all cells was stained blue with DAPI. Numerous green-stained neurons were observed in the CLP and CLP + H2 + GW6471 groups vs. the CLP + H2 group, which were significantly decreased by 2% H2 inhalation close to PPARα. Scale bar = 50 μm. *P < 0.05 vs. Sham group; #P < 0.05 vs. CLP group; &P < 0.05 vs. CLP + H2 group

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BMC Neuroscience

ISSN: 1471-2202

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