Fig. 1

Experimental design. (A) C57BL/6J mice (6–8 w, 20–25 g) were subjected to sham or CLP operation. One hour after the injection of GW6471 or vehicle, sham and CLP operations were conducted. H2 or fresh air was inhaled for 60 min starting from 1 and 6 h postoperation with H2 concentration detection. The brain tissue of different groups was obtained for tests 24 h after the sham or CLP procedure. Memory cognitive function tests were conducted from 24 h to 7 days after the sham or CLP procedure. Different groups of brain tissues were used for all the examinations as described in the Materials and Methods. (B) Mouse bEnd.3 cells were incubated with control + DMSO or GW6471 medium, control + HW + DMSO or GW6471 medium, LPS + DMSO or GW6471 medium, and LPS + HW + DMSO or GW6471 medium. The cells and culture medium supernatant were collected for testing 24 h after incubation. CLP, caecal ligation and puncture; LPS, lipopolysaccharide; GW6471, a PPARα inhibitor