Fig. 3

EPO is a positive effector of neuronal differentiation in female NCSCs. A: Immunocytochemistry revealed a slightly reduced expression of synaptophysin in male NCSCs after 3 weeks of neuronal differentiation supplemented with 1 U/ml EPO. White arrows depict synaptophysin positive presynapsis in female neurons. B, C: Camera Lucida Tracing and quantitative analysis reveals elongated axons of female NCSCs upon EPO supplementation (1 U/ml) during 10 weeks of neuronal differentiation. Mann-Whitney test, *p < 0.05, **p < 0.01, ***p < 0.001. was considered significant