Fig. 2From: SUFI: an automated approach to spectral unmixing of fluorescent multiplex images captured in mouse and post-mortem human brain tissuesExtracted spectral signatures (fingerprints) from mouse and post-mortem human DLPFC data. Spectral signatures are extracted from single positive slides using the Vector Component Analysis (VCA). In each subplot, normalized pixel intensity is plotted against wavelength. (i) Solid lines represent the fingerprints extracted manually using ZEN Black software. (ii) Dotted lines with bubbles represent fingerprints extracted automatically using VCA. The color corresponds to peak wavelength for DAPI and Opal dyes. Lipofuscin is pseudo-colored to black. Root mean squared error (RMSE) between the two lines is calculated for each set of fingerprintsBack to article page