Fig. 4From: Histological characterization of orphan transporter MCT14 (SLC16A14) shows abundant expression in mouse CNS and kidneyMCT14 is expressed in the soma of neurons and in epithelial cells. Fluorescent double IHC on paraffin sections from wild-type mouse brain with the MCT14 antibody and a panel of markers to identify the cell types expressing MCT14. The MCT14 antibody staining is seen in red, cell-specific markers are seen in green, and cell nuclei stained with DAPI are seen in blue. a Colocalization of MCT14 with the whole-neuron marker Pan neuronal cocktail aimed at soma, dendrites, axons and nuclei of neurons suggests that MCT14 is expressed in the neuronal soma in cortex. b Reduced MCT14 staining following pre-incubation of anti-MCT14 with a peptide corresponding to the antibody epitope, while Pan still stains the whole neuron. c No overlap between MCT14 and the astrocytic marker GFAP. d Overlap between MCT14 and neuronal marker MAP2 in the soma of neurons. e No colocalization of MCT14 and the pre-synaptic vesicle marker synaptophysin. f Colocalization of MCT14 and Pancytokeratin, an epithelial cell marker, indicates MCT14 expression in epithelial cells of the choroid plexus in the lateral ventricles. All images were taken at ×40 magnification on a Zeiss AxioImager, and all scale bars represent 10 µmBack to article page