Table 1 Nucleotide sequences of primers for real time quantitative polymerase chain reaction
Gene | Forward primer | Reverse primer |
|---|---|---|
TLR4 | 5′-GAGGTTGCTGTTCTTATTCTGAT-3′ | 5′-GAGTGCTGAAAGTCCAGGTATT-3′ |
MYD88 | 5′-GAAATACATACGCAACCAGCAGAAA-3′ | 5′-CAGATGAAGGCGTCGAAAAGC-3′ |
NF-kB | 5′-GACCTGGAGCAAGCCATTAGC-3′ | 5′CGGACCGCATTCAAGTCATAGT-3′ |
IL-1β | 5′-GTGGTATTCTCCATGAGCTTTGTA-3′ | 5′-CCATCTTCTTCTTTGGGTATTGTT-3 |
β-actin | 5′-TGAGACCTTCAACACCCCAG-3′ | 5′-GCCATCTCTTGCTCGAAGTC-3′ |