Fig. 4

Indomethacin, administered prior to pentylenetetrazole-induced seizures, on cox2b level in the brain of zebrafish. Relative quantification of cyclooxygenase 2 b (cox2b) mRNA expression level 0.05 h after pentylenetetrazole (PTZ)-induced seizures in the brain of zebrafish at 7 days post fertilization. Seizure group (SG) was composed of animals exposed to 15 mM PTZ for 20 min. Indomethacin groups (10, 100, and 307 μM) were composed of animals that received indomethacin treatment prior to PTZ. Animals in the control group (CG) were handled identically, but they were treated with water (no PTZ and no indomethacin treatments; n = 5 per group). Data are presented as mean ± SEM. Statistical analysis was performed by one-way analysis of variance (ANOVA) with Bonferroni’s post hoc test. One asterisk (*) indicated that p ≤ 0.05; two asterisks (**) indicated that p ≤ 0.01; three asterisks (***) indicated that that p ≤ 0.001. CG control group, SG seizure group