Table 3 Antibodies used in this study

Anti-UbcM2

Recombinant His₆-S-tagged unique N-terminal extension (MSSDRQRSDDESPSTSSGSSDADQRDPAAPEPEEQEERKPSATQQKKNTKLSSKTTAK)

In-house, rabbit, polyclonal

1:500 (IHC)/1:1000 (WB)

Anti-Cre recombinase

aa 77-343 fusion protein

EMD Millipore, mouse, monoclonal, MAB3120, RRID:AB_2085748

1:2000

Anti-GFAP

Recombinant GFAP, boosts with GFAP purified from bovine spinal cord

Neuromics, mouse, polyclonal, MO22136, RRID:AB_2341541

1:500

Anti-myelin CNPase

Purified human myelin CNPase

Covance, mouse, monoclonal, SMI 91, RRID:AB_10120658

1:2500

Anti-Iba1

Linear peptide corresponding to human Iba1/AIF1

EMD Milipore, mouse, monoclonal, MABN92, RRID:AB_10917271

1:200

Anti-NeuN

Purified cell nuclei from mouse brain

EMD Millipore, mouse, monoclonal, MAB377, RRID:AB_2298772

1:2000

Anti-HSP70

Native human HSP70

Enzo, mouse, monoclonal, ADI-SPA-810, RRID:AB_10616513

1:1000

Anti-ubiquitin

Amino acids 1-76 representing full length ub of bovine origin

Santa Cruz Biotechnology, mouse, monoclonal, sc-8017, RRID:AB_628423

1:1000 (WB)/1:500 (IHC)

Anti-β-tubulin

Tubulin from rat brain

Sigma-Aldrich, mouse, monoclonal, T4026, RRID:AB_477577

1:1000