Enhanced nigrostriatal neuron-specific, long-term expression by using neural-specific promoters in combination with targeted gene transfer by modified helper virus-free HSV-1 vector particles

Table 1 The numbers of β-gal or PkcΔGG positive cells that contained TH-IR, in rats sacrificed at 4 days after co-injection of pTHlac/gC – bdnf and pHSVpkcΔGG/gC – bdnf into the midbrain

	Numbers of positive cells		%	Numbers of positive cells		%
Rat	β-gal-IR	TH-IR	Costained	Flag-IR	TH-IR	Costained
1	347	287	83	328	232	71
2	322	278	86	205	134	66
3	340	274	81	470	354	75
4	418	358	86	310	212	68
Average			84 ± 1			70 ± 2

A mixture of equal IVP/ml of pTHlac/gC – bdnf and pHSVpkcΔGG/gC – bdnf (for titers and injection conditions, see methods) was injected into the midbrain proximal to the substantia nigra. The rats were perfused at 4 days after gene transfer, the brains were sectioned, and alternating sections were costained for either β-gal-IR or flag-IR and TH-IR. Sufficient sections were examined to count at least 200 cells that contained either β-gal-IR or flag-IR in each rat. In the sections that were examined, all of the β-gal-IR or flag-IR cells were scored for containing or lacking TH-IR. For the average % costaining, the means ± sems are shown.

ISSN: 1471-2202