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Table 1 List of proteins identified in synaptosomes and crude cytosol from hippocampal culture or tissue

From: Protein fingerprints of cultured CA3-CA1 hippocampal neurons: comparative analysis of the distribution of synaptosomal and cytosolic proteins

Spot number

Accession number*

Protein name

S tis

S cul

C cul

Method of detection

Cytoskeletal and their interacting Proteins(CK)

4

P60711

Actin, cytoplasmic

x

x

x

2DE-MS

37

P63259

Actin, cytoplasmic 2

x

x

x

2DE-MS

48

Q61274

Alpha-cardiac actin [Fragment]

x

x

 

2DE-MS

100, 101, 123

P68370

Tubulin alpha-1 chain

x

x

x

2DE-MS

36, 39, 47,124

P04691

Tubulin beta chain

x

x

x

2DE-MS

22

P37805

Neuronal protein 25

x

x

 

2DE-MS

53

P13668

Stathmin

x

x

x

2DE-MS

119

P13668

Stathmin

  

x

2DE-MS

55

Q63228

Glia maturation factor beta

x

x

x

2DE-MS

33

P45592

Cofilin, non-muscle isoform

x

x

x

2DE-MS

92

Q9R0P5

Destrin

x

x

x

2DE-MS

50

P63029

Translationally controlled tumor protein

x

  

2DE-MS

125

P62963

Profilin I

 

x

x

2DE-MS

127

Q9EPC6

Profilin II

 

x

x

2DE-MS

Metabolism(M)

18, 105, 106

P04797

Glyceraldehyde-3-phosphate dehydrogenase

x

x

x

2DE-MS

71

P16617

Phosphoglycerate kinase, testis specific

x

x

x

2DE-MS

96

P10719

ATP synthase beta chain, mitochondrial

x

x

x

2DE-MS

11

P31399

ATP synthase D chain, mitochondrial

x

x

 

2DE-MS

26, 27

P04764

Alpha enolase

x

x

x

2DE-MS

84

P11980

Pyruvate kinase, M2 isozyme

x

x

x

2DE-MS

78

Q9ER34

Mitochondrial aconitase [Precursor]

x

x

x

2DE-MS

2, 67

P10860

Glutamate dehydrogenase, mitochondrial [Precursor]

x

x

x

2DE-MS

34, 35, 64

P04636

Malate dehydrogenase, mitochondrial [Precursor]

x

x

 

2DE-MS

49

P11240

Cytochrome c oxidase polypeptide VA

x

x

x

2DE-MS

8, 85

Q99NA5

NAD+specific isocitrate deihydrogenase a-subunit

x

x

 

2DE-MS

83

P42123

L-lactate dehydrogenase B chain

x

x

x

2DE-MS

5

P48500

Triosephosphate isomerase

x

x

x

2DE-MS

31, 103

P05065

Fructose-biphosphate Aldolase A

x

x

x

2DE-MS

40

P09117

Fructose-bisphosphate aldolase C

x

x

x

2DE-MS

41, 68

Q9D0K2

Succinil-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial

x

x

x

2DE-MS

56

Q05982

Nucleoside diphosphate kinase A

x

x

x

2DE-MS

61

P19804

Nucleoside diphosphate kinase B

x

x

x

2DE-MS

69

P09606

Glutamine synthetase

x

x

x

2DE-MS

70

P25809

Creatine kinase, ubiquitous mitochondrial [Precursor]

x

 

x

2DE-MS

75

P13221

Aspartate aminotransferase cytoplasmatic

x

x

x

2DE-MS

122

P07335

Creatine kinase, B chain

x

x

x

2DE-MS

1, 82

O88989

Cytosolic malate dehydrogenase

x

x

x

2DE-MS

80

P97532

3-mercaptopyruvate sulfurtransferase

x

  

2DE-MS

90

P04764

Alpha-enolase

x

 

x

2DE-MS

104

Q80YG1

Phosphoserine aminotransferase

 

x

x

2DE-MS

113, 114

P48500

Triosephosphate isomerase

 

x

x

2DE-MS

16

P19234

NADH-ubiquinone oxidoreductase 24 kDa subunit

x

x

x

2DE-MS

Synaptic components (SY)

72, 76, 86, 87

P47942

Dihydropyrimidinase related protein-2 (drp-2)

x

x

x

2DE-MS

89

Q62952

Dihydropyrimidinase related protein-3 (drp-3)

x

x

x

2DE-MS

3

Q63537

Synapsin II

x

x

x

2DE-MS

29

P07825

Synaptophysin

x

x

 

WB

30

P32851

Syntaxin 1 A-E

x

x

 

WB

46

P37377

Alpha-synuclein

x

x

 

2DE-MS

45

Q63754

Beta-synuclein

x

x

 

2DE-MS

73

P25286

Vacuolar proton translocating ATPase subunit a isoform 1

x

x

 

2DE-MS

9, 51

P31044

Phosphatidylethanolamine-binding protein 1

x

x

x

2DE-MS

19

P55051

Fatty acid-binding protein, brain

x

x

x

2DE-MS

126

P11030

Acyl-CoA-binding protein

 

x

x

2DE-MS

128

P62775

Myotrophin

  

x

2DE-MS

Receptors, Ion Channel and adhesion molecules (I)

23, 63

Q6P9W9

Voltage-dependent anion-selective channel protein 1

x

x

x

2DE-MS

66

P50516

Vacuolar ATPsynthase catalytic subA

x

x

 

2DE-MS

Vesicle Transport and Recycling (T)

97

P46462

Transitional endoplasmic reticulum ATPase

x

 

x

2DE-MS

25

P63012

Ras-related protein rab-10

x

x

x

WB

28

Q62824

Exocyst complex component Sec8

 

x

 

WB

Protein Fate (synthesis, folding, modification and destination) (PF)

38

P63039

60 kda heat shock protein, mitochondrial precursor (hsp60)

x

x

x

2DE-MS

65

P63017

Heat shock 70 kDa protein-ps1

x

x

x

2DE-MS

102

P07901

Heat shock protein [Fragment]

  

x

2DE-MS

44

P48721

Stress-70 protein, mitochondrial [Precursor]

x

x

x

2DE-MS

17

Q00981

Ubiquitin carboxyl-terminal hydrolase isozyme L1

x

x

 

2DE-MS

15

P20108

Mitochondrial thioredoxin-dependent peroxide reductase

x

x

x

2DE-MS

52

Q9DB15

39S ribosomal protein L12, mitochondrial

x

x

 

2DE-MS

6

P52555

Endoplasmic reticulum protein ERp29

x

x

x

2DE-MS

32, 98, 99

P10111

Peptidyl-prolyl cis-trans isomerase A

x

x

x

2DE-MS

42, 43, 79

P11598

Protein disulfide isomerase A3

x

x

x

2DE-MS

77

O35814

Stress induced phosphoprotein 1

x

x

x

2DE-MS

88

P11983

T complex polypeptide 1b

x

x

x

2DE-MS

21

P55053

Fatty acid-binding protein, epidermal

x

x

x

2DE-MS

59

P07483

Fatty acid-binding protein, heart

x

x

x

2DE-MS

57

Q9EQX9

Ubiquitin-conjugating enzyme e2

x

x

x

2DE-MS

108, 109

P04905

Glutathione S-transferase Yb-1

  

x

2DE-MS

107

P04904

Glutathione S-transferase Yc-1

  

x

2DE-MS

112

P08010

Glutathione S-transferase Yb-2

  

x

2DE-MS

111

P08009

Glutathione S-transferase Yb-3

  

x

2DE-MS

116

P14942

Glutathione S-transferase alpha-4

  

x

2DE-MS

110

Q63716

Peroxiredoxin-1

 

x

x

2DE-MS

54

P35704

Peroxiredoxin-2

x

x

x

2DE-MS

93

Q9R063

Peroxiredoxin-5, mitochondrial [Precursor]

x

x

x

2DE-MS

94

Q63347

26S protease regulatory subunit 7

  

x

2DE-MS

115

P17220

Proteasome subunit alpha type 2

 

x

x

2DE-MS

91

Q9JHW0

Proteasome subunit beta type 7 [Precursor]

x

 

x

2DE-MS

Signalling Proteins (S)

24

P10354

Chromogranin A

x

x

 

WB

74

P50399

RAB GDP dissociation inhibitor beta-2

x

x

x

2DE-MS

10

P60766

Cell division control protein 42 homolog

x

x

 

2DE-MS

60

P70349

Histidine triad nucleotide-binding protein

x

x

x

2DE-MS

81

P14669

Annexin III

x

  

2DE-MS

117

Q62658

FK506-binding protein 1°

 

x

x

2DE-MS

120

P62260

14-3-3 protein epsilon

 

x

x

2DE-MS

121

P63102

14-3-3 protein zeta/delta

 

x

x

2DE-MS

62

P62994

Growth factor receptor bound protein 2

x

x

 

2DE-MS

Other (O)

12

O35264

Platelet-activating factor acetylhydrolase IB subunit beta

x

 

x

2DE-MS

13

P67779

Prohibitin

x

x

x

2DE-MS

7

O35244

Antioxidant protein 2

x

x

x

2DE-MS

20

P07632

Superoxide dismutase [Cu-Zn]

x

x

x

2DE-MS

58

Q9CPT4

IL25

x

x

x

2DE-MS

14

O88767

CAP1 PROTEIN

x

x

x

2DE-MS

118

P01041

Cystatin B

  

x

2DE-MS

95

Q8CDL9

Similar to hypothetical protein

x

x

x

2DE-MS

  1. S: synaptosome; C: crude cytosol; cul: culture; tis: tissue; 2DE-MS: two-dimensional electrophoresis and MALDI-TOF mass spectrometry; WB: Western blot; * Swiss-Prot database; Spot numbers correspond to the spot numbers indicated in Fig. 1 and 4.
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BMC Neuroscience

ISSN: 1471-2202

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