Figure 2From: The Kv2.1 K+ channel targets to the axon initial segment of hippocampal and cortical neurons in culture and in situThe single proximal neurite containing Kv2.1 corresponds to the axon initial segment. Hippocampal neurons grown for 10 days were transfected with EGFP-Kv2.1-HA and fixed 18 h later. Cultures were then labeled with either anti-MAP2 or anti-AnkG followed by Alexa 594-conjugated secondary antibodies. Shown in panel A is an example of Kv2.1 clustering in the proximal axon as indicated by the diminished MAP2 staining. Panel B illustrates intense AnkG staining in the Kv2.1 positive process. The right hand panels contain enlarged images of the neurites boxed in Panels A and B. Note that the diffuse GFP signal in the neurites represents intracellular GFP-Kv2.1.Back to article page