Figure 9

12 μm sagittal sections of the caudate putamen in a two-month-old rat were processed for immunohistochemistry. The sections were incubated with anti Necl-3 and anti GFAP antibodies (A, B, C, 14 z-stacks of 0.12 micron each) or with anti Necl-3 and anti CD11b antibodies (D, E, F, 20 z-stacks of 0.36 micron each), which were revealed with secondary antibodies as in Fig. 5. (A), GFAP immunoreactivity in the green-pass channel; (B), Necl-3 immunoreactivity in the red-pass channel; (D), CD11b immunoreactivity in the green-pass channel; (E), Necl-3 immunoreactivity in the red-pass channel. The green and red images are merged in (C) and (F). Nuclei were visualized with DAPI in (A), (B), (D), and (E).