Figure 5

Muskelin is localized in nuclei, cell somata and neurite processes. (A1) Muskelin immunoreactive signals are strong in stratum pyramidale (SP) of the hippocampus containing cell bodies of CA1 pyramidal cells. Note that muskelin displays also punctate localizations in stratum oriens (SO) and stratum radiatum (SR), containing basal and apical dendrites of pyramidal cells, respectively. (A2) Muskelin immunoreactive signals are strong in the dentate gyrus granule cell layer (GCL) containing cell bodies of granule cells. A punctate muskelin localization is also found in the molecular layer (ML) harbouring granule cell neurite processes. Note, that also cells in the molecular layer, most likely representing interneurons (arrowheads), express muskelin. (B1, B2) In cerebellum, prominent staining is found in the Purkinje cell layer (PCL). High power magnification reveals that muskelin puncta are detectable in nuclei and cell bodies of Purkinje cells located in the Purkinje cell layer (PCL), as well as in the molecular layer (ML) harbouring Purkinje cell dendrites and parallel fiber axons of granule cells. Also in the granule cell layer (GCL) muskelin localizes in a punctate distribution in cell somata and neurites. Scale bars: 50 μm.