Figure 3From: Regulation of nestin expression by thrombin and cell density in cultures of bone mesenchymal stem cells and radial glial cellsExpression of the PAR1 thrombin receptor in RG and rat MSCs cultures. RG and rat MSCs were cultured for 2 days in the presence of serum (A, B, E) or were maintained for the same time in serum-free medium (C, D, F). Then, the cells were fixed and RG were labelled with anti-nestin (green), anti-PAR1 (red) and anti-GFAP (blue) antibody (panel A-D) while rat MSCs were stained with anti-nestin (green) and anti-PAR1 (red) antibody (panel E, F). Scale bar (in A = 10 μm) applies to all images. Expression of thrombin receptor PAR1mRNA in mouse RG (G) and rat MSCs and astrocytes (H) was analyzed by RT-PCR using optimized primers for each species.Back to article page