Figure 1
From: Stable silencing of SNAP-25 in PC12 cells by RNA interference
Plasmid-based expression of shRNA-SNAP-25. A) Plasmid map of pG418-shRNA, a plasmid designed to express shRNAs from the mouse U6 promoter. B) The two 56-base deoxyoligonucleotides used in the construction of pG418-shRNA-SNAP-25 are shown as they would be paired after annealing. The labels above and below the oligonucleotides indicate the source or the function of the nucleotides in the indicated regions. C) The predicted stem-loop structure of shRNA-SNAP-25 expressed from pG418-shRNA-SNAP-25.