Figure 1From: Glutamate-induced apoptosis in neuronal cells is mediated via caspase-dependent and independent mechanisms involving calpain and caspase-3 proteases as well as apoptosis inducing factor (AIF) and this process is inhibited by equine estrogensExpression of estrogen receptors (ERα and ERβ) mRNA and protein in rat neuronal cortical cells. Rat cortical cells were cultured for 7–8 days and total RNA and protein were extracted. RT-PCR and Western blot analysis were carried out as described under "Methods". Rat uterus was used as a positive control.Back to article page