Figure 3

Effects of laser surgery on cryophilic bias. The amount of cryophilic bias exhibited by worms assayed above and below T _cult . In each panel, black bars correspond to experiments with worms that did not undergo surgery or surgical preparation, gray bars correspond to experiments in which AFD dendrites were severed, and white bars correspond to experiments in which neurons were killed. Mock control measurements represent worms that were prepared for surgery without undergoing laser irradiation. The values of the thermotactic index are means ± one standard error. The cases in which surgery significantly affects cryophilic bias in comparison to its respective mock control are indicated by asterisks (***, P < 0.0005; *, P < 0.05). All other cases are not distinguishable from their respective mock controls (P > 0.2). Significance was measured using a two-tailed Student's t-test. The number of worms tested in each measurement of cryophilic bias is noted next to each data bar.