Table 6 Primer sequences
From: Sex-dependent gene expression in early brain development of chicken embryos
Gene | Amplicon (nt) | Forward (5'-3') | Reverse (3'-5') |
|---|---|---|---|
AATF | 64 | TTACCGCTCACTGTTTGGAAGA | TCCCGGATGCCAACCA |
ACAA2 | 76 | TCTGGTTTCCAGTCCATTGCT | CTCCACCACACAGAACCACTTC |
ACAD8 | 70 | CATGGCCAAGCTGTTTGCTA | GCCCCCGTGCATCTGTAG |
ANXA1 | 78 | TGAATGATGATCTTGCTGACAATG | CGTTAACATCCGTCCCTTTCC |
BTF2-P44 | 85 | CCTCCACCTGCCAGTTCAAC | CCTGGTCAGAAAGGGAAGCA |
CAT1 | 71 | CACGCTGATGGTGCCCTACT | AGCCCACCGCCTTGAAAG |
CCNH | 73 | AACCGAAAGTTTCGCAGCAA | GGGCCTCCAGCAGGAAAG |
CDK7 | 78 | GGGACAGATCGTGGCTATCAAA | TGAGAGCTGTTCTGTTGATTCCA |
CETN3 | 77 | GAAATACTCAAGGCGTTCAAATTGT | GCAACCCGACGCAGGTT |
CEZANNE2 | 67 | CCACAACTGGTGATGGAAACTG | CGGTCATGAAATCCCCACAT |
CEZANNE2 e! | 69 | GGCCACAACTGGTGATGGA | CGGTCATGAAATCCCCACAT |
CLA | 51 | GTAAGTGGAGAGAGGAGCAAAAGG | AGTTTGCATCAAGCTGCTCAAG |
CX3CR | 74 | CACCGTGTCTGCTTCAAGTACTG | TATGTGCGGACGTCATAATGATT |
DHFR | 79 | ATGGGCATCGGGAAGGA | TGCTGGTCATTCTCTGGAAGTACT |
DMRT1 | 81 | GAAGCCTCCCAGCAACATACA | CATGGCATGGCGGTTCTC |
HERP1/HEY2 | 68 | GCGACCGGAGGTAAAGGTTATT | CGGAAGCCAATGCTCATAAAA |
HINT | 77 | ATATTCTGGGAGGTCGTCAGTTG | TTGTACACATGCAGCATCTCTTGT |
HMGCOA | 84 | GAAGAAAATAAACCAAACCCTGTAACA | GCGACTGTGTGCATGAACAAG |
MAT1 | 62 | CCCCTCCCTGAAGCTGATG | GCTCCACGCAGCTCTCACA |
MHM array clone | 76 | CAACAGCACTACTGGAGCCATATG | GGAAGCCCCTGCAAAGAAG |
MHM GenBank | 69 | GAAATGCCTCGCCCTTTAAGT | TGACTGCCCTTGGTTGTCTGT |
MRPL3 | 72 | GAGTTGGTGTTGTTGCGGTAA | TGACAGCGTGCTTCTTTCCT |
NAT5 | 51 | CTGACGGAGACCTACGGCAT | CCAGTGTGCCAGGTACTGCA |
P311 | 75 | TCATGGTTTCCTTGTGGTAAGTGA | TCCAAAATAGCACCATTCATCAGT |
PRTD-NY3 | 68 | TCACACGGTCTCCATACCTCAA | CCGACAGCCTGCCATCTC |
RJA094A01 | 74 | GGGAGGGTGGATCTGTGACA | ACAGCCCTTCCCGTAAAAGAG |
SMAD2 | 51 | AGTGGGACACAACAGGCCTT | CGAGAGATCTGGTTTGTTCAGAGAA |
SOLT | 61 | TTATTAGGAGAAGAAGAGTTGCAGAAAG | CTGCTGACAGCACCATTTCAA |
SPINW | 102 | CTGTTCAAGAATAAATAGAATCACAATGG | TTCAGTCCTCACATAGTCCAACTTAGTT |
SPINZ | 101 | CCAAGTTCCTGTAAATCCCTCTCTT | CAAGCGCAGAAACTCTTTCATCT |
TINP1 | 77 | AAGCTGGGAAATGGGAGGTT | AAGCTGGGAAATGGGAGGTT |
TXN | 72 | TGATGTGGTGTTCATTGAAATTGA | GGCATGCACTTCACATCACAGT |
UBE2R2 | 76 | CCAAAGCTGAGGCAGAAAAGG | GGCACTTTAGTTTTGATGCAGTACTC |
WLA084D05/ABWT | 72 | TGGGATGGCTGCATGTGTA | AACCCCGCCACTGGTATTC |
WPKCI-8 | 51 | AGATTGTGGCGCACCTCTTC | CACTTCTCGCCAACAATCATCA |
ZNF216 | 67 | GCCCCGGCCCCATT | GCATTAAAACTACAGAATCCTCTTTGC |
ZO-2 | 83 | CCTTTTCTTCCCCTTTTATTAATGCT | TCTGTTGCATCAAGTTTATCTCCTAGA |
ZOV3 | 86 | CGATACCTGCCTCCAGTGTGT | GAGGAACAGACCTATTTACAAACTGAAA |