Figure 2

Hsp27 co-localizes with actin and tubulin at early stages of neurite growth. Neurons were plated on polylysine and stimulated with laminin for 1–6 hrs. Following fixation, neurons were labelled with rhodamine-phalloidin (red-A, D) or immunostained with antibodies directed against total tubulin (red – G, J) or Hsp27 (green -B, E, H, K). Images were obtained with confocal microscopy and panels C, F, I, L represent the merged images of the single channel images. Note colocalization of Hsp27 and actin in the lamellopodium (A-C, arrow) and in focal contacts observed in D-F (arrow). In panels G-I, there is some colocalization of the staining for tubulin and Hsp27 in the cortical region (large arrowhead) and in small processes emerging from the soma (arrow). In panels J-L, there is a more distinct colocalization of tubulin and Hsp27 in the cortical area (arrow, J-L) as well as in an obvious process that seems to be wrapping around the cell and finally extending (arrowhead, J-L). Scale bar – 20 μm