Figure 3From: Valine 738 and lysine 735 in the fifth transmembrane domain of rTas1r3 mediate insensitivity towards lactisole of the rat sweet taste receptorEffect of lactisole on Tas1r3 variants. (a, b) Responses of cells co-transfected with rTas1r2 and rTas1r3 mutants to stimulation with 1 mM stevioside. (c) Calcium responses of cells co-transfected with rTas1r2 and the rTas1r3-V738A receptor variant to stimulation with various sweeteners in the presence and absence of lactisole. c, response of mock-transfected control cells; lac, 1.0 mM lactisole; asp, 15 mM aspartame; cyc, 30 mM cyclamate; neo, 0.1 mM neohesperidine dihydrochalcone; sac, 10 mM saccharin; suc, 100 mM sucrose; stev, 1 mM stevioside; -, response without lactisole; +, response in the presence of 1.0 mM lactisole. All sweeteners were used at concentrations which were close to the maximal obtainable signal. (d) Concentration-dependent inhibition of calcium responses by lactisole in cells transfected with hTAS1R2-hTAS1R3 (open circles, solid line), rTas1r2-rTas1r3 V738A (squares, dash-dot-dotted line), rTas1r2-rTas1r3 m6 (open triangles up, long dashed line), rTas1r2-rTas1r3 m6/1 (filled circles, short dashed line), rTas1r2-rTas1r3 m6/2 (triangles down, medium dashed line) or rTas1r2-rTas1r3 m6/3 (open diamond, dotted line) that were challenged with 3 mM stevioside.Back to article page