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Table 1 Sense and anti-sense oligonucleotides for Smads 2, 3 and 4

From: TGF-β1 induction of the adenine nucleotide translocator 1 in astrocytes occurs through Smads and Sp1 transcription factors

Gene

Sequence (5'-3')

Position

Annealing temp (°C)

Smad2

TCAGTGCGATGCTCAAGCATGTCC

1515-1538

66

 

AGACCAAGCAGCAGCACACACCTC

1926-1903

 

Smad3

ACGAGCTGAACCAGCGAGTTGG

1006-1027

45 (5 cycles)

 

TGGTGCACATGCGAGTCAACTGG

1407-1385

57 (35 cycles)

Smad4

ATGCCCATTGCGGACCCACAG

1755-1775

66

 

GGAAGACTGGACTGCCACTTGCG

2253-2231