Channel | Inactivation | n |
---|
| V50inact (mV) | kinact | |
---|
CaV2.1/β4/α2δ2 | -31.52 ± 1.58 | 7.04 ± 0.19 | 18 |
CaV2.1/β4/α2δ2/γ2 | -33.44 ± 1.49 | 7.10 ± 0.23 | 18 |
CaV2.1/β4/α2δ2 | -31.40 ± 0.89 | 7.04 ± 0.28 | 20 |
CaV2.1/β4/α2δ2/γ4 | -34.60 ± 1.43 | 6.61 ± 0.28 | 20 |
CaV2.1/β4 | -32.40 ± 1.09 | 8.43 ± 0.61 | 22 |
CaV2.1/β4/γ2 | -37.30 ± 1.75 | 7.52 ± 0.74 | 16 |
CaV2.1/β4/γ4 | -31.50 ± 1.80 | 8.40 ± 0.92 | 9 |
- Steady-state inactivation data from Figures 7C, 7D and 7E were fitted with a single Boltzmann function (see methods). Values are expressed as mean ± S. E. M. of the number of replicates, n. In the presence of an α2δ2 subunit, neither γ2 nor γ4 caused statistically significant modulation of the measured parameters according to an un-paired Student's t-test (P < 0.05 was considered significant). Equally, when the α2δ2 subunit was omitted from the injection mixture, ANOVA determined no statistically significant changes in any pairs of data differing by the presence of a γ protein.