Channel | Activation | | n |
---|
| V0.5act(mV) | kact. | Gmax (μS) | Peak at +10 mV (μA) | |
---|
CaV2.1/β4 | -2.60 ± 0.65 | 5.39 ± 0.49 | 8.45 ± 2.20 | -0.25 ± 0.05 | 16 |
CaV2.1/β4/γ2 | -4.27 ± 1.24 | 4.91 ± 0.52 | 9.96 ± 3.34 | -0.32 ± 0.12 | 14 |
CaV2.1/β4/γ4 | -5.70 ± 2.26 | 5.43 ± 0.47 | 8.16 ± 2.59 | -0.26 ± 0.07 | 11 |
CaV2.1/β4/α2δ2 | -4.60 ± 0.59 | 4.03 ± 0.15 | 14.97 ± 2.05 * | -0.54 ± 0.08 | 43 |
CaV2.1/β4/α2δ2/γ2 | -4.13 ± 0.63 | 4.46 ± 0.14 | 15.43 ± 1.96 | -0.54 ± 0.07 | 34 |
CaV2.1/β4/α2δ2/γ4 | -5.56 ± 0.60 | 3.96 ± 0.24 | 14.8 ± 2.64 | -0.56 ± 0.10 | 20 |
- To analyze the voltage-dependent activation, current-voltage (I-V) relationships (Figure 7A and Figure 7B) were fitted with a modified Boltzmann equation (see methods) to calculate V50act, the mid-point of voltage dependence of activation, kact, the slopefactor and Gmax, the maximum conductance. Values are expressed as mean ± S. E. M. of the number of replicates, n. One-way analysis of variance (ANOVA) tests determined no statistically significant changes in any pairs of data differing by the presence of a γ2 or γ4 P < 0.05 was considered significant). Statistically significant differences observed between the CaV2.1/β4 and CaV2.1/β4/α2δ2 channels are indicated by * (P < 0.05).