From: Estrogen protects against the synergistic toxicity by HIV proteins, methamphetamine and cocaine
Antigen | Primary | Dilution | Antibody | Secondary | Specificity & |
---|---|---|---|---|---|
 | antibodies/ |  | Source | antibodies/antisera | Subcellular distribution |
 | antisera |  |  | (dilutions) |  |
Estrogen | Mouse | 1:10 | Chemicon | Goat anti-mouse IgG | Neurons and astrocytes / |
receptor | IgG1 | Â | (MAB428) | (H+L)conjugated to Cy3 | Punctate nuclear and |
(ER) | monoclonal | Â | Â | (1:250) | perinuclear distribution |
Dopamine | Rabbit | 1:1000 | Chemicon | • Goat anti-rabbit IgG | Neuronal processes and cell |
(DA) | IgG | Â | (AB122S) | DTAF (1:500) or | body |
 | polyclonal |  |  | • Goat anti-rabbit IgG | Cytoplasmic distribution |
 |  |  |  | Cy3 (1:250) |  |
Dopamine | Rat | 1:100 | Chemicon | Goat anti-rat IgG | Neuronal processes and cell |
transporter | Ig type | Â | (MAB369) | conjugated to biotin- | body; astrocytes |
(DAT) | monoclonal | Â | Â | avidin-Cy2 (1:250) | Cell membrane |
Dopamine | Rabbit | 1:100 | Chemicon | Goat anti-rabbit IgG | Neurons and astrocytes; |
D1A | polyclonal | Â | (AB1784P) | DTAF (1:500) | punctate distribution |
receptor | Â | Â | Â | Â | Â |
Dopamine | Rabbit | 1:500 | Chemicon | Goat anti rabbit IgG | Neuronal processes and cell |
D2 | polyclonal | Â | (AB1558) | DTAF (1:500) | bodies: perinuclear |
receptor | Â | Â | Â | Â | distribution, astrocytes, |
 |  |  |  |  | microglia |
Co-localization | |||||
Combined |  |  |  | • Goat anti-rabbit IgG | Colocalized in neurons |
• DA + | as above | as above | as above | DTAF + | and/or astrocytes |
• ER |  |  |  | • Goat anti-mouse |  |
 |  |  |  | Cy3 |  |
Combined |  |  |  | • Goat anti-rabbit IgG | Colocalized in |
• DA + | as above | as above | as above | Cy3 | subpopulations of neurons |
• DAT |  |  |  | • Goat anti-rat IgG | and/or astrocytes |
 |  |  |  | biotin-avidin Cy2 |  |
Combined | as above | as above | as above | • Goat anti-rabbit IgG | Colocalized in |
• D1A + |  |  |  | DTAF (1:500) | subpopulations of neurons |
• ER |  |  |  | • Goat anti-mouse | and/or astrocytes |
 |  |  |  | Cy3 (1:250) |  |
Combined | as above | as above | as above | • Goat anti-rabbit IgG | Colocalized in |
• D2 + |  |  |  | DTAF (1:500) | subpopulations of neurons |
• ER |  |  |  | • Goat anti-mouse | and/or astrocytes and |
 |  |  |  | Cy3 (1:250) | microglia |